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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Molecular breeding 1 (1995), S. 419-423 
    ISSN: 1572-9788
    Keywords: binary vectors ; gus ; T-DNA ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We describe here a set of binary vectors suitable forAgrobacterium-mediated gene transfer and specially designed for studying plant promoters. These vectors are based on the use of thegus reporter gene, contain multiple unique restriction sites upstream of thegus gene, and minimal promoters for testing the effect of enhancers or activator elements. In addition, an intron-containinggus (uidA) gene was introduced into one of these vectors in order to examine reporter gene activity in tissues whereAgrobacterium contamination may be a problem or in transient expression assays.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 137-149 
    ISSN: 1573-0972
    Keywords: Chitinase ; fatty acid synthesis ; lipochitooligosaccharide signal ; NodA ; nodulation genes ; root development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract During the past five years the structure of nodulation signals from more than a dozen different Rhizobium species has been elucidated. In addition, the role of numerous nod genes in the biosynthesis of the lipooligosaccharides has been identified. This review discusses how Nod signal structure is determined by the specificity of the various biosynthetic steps and how this influences variation in host specificity. Until recently, it appeared that the decorations of a common lipochitooligosaccharide core determine the host-specific recognition of the signals, possibly via specific receptors in the host plant cell. A number of recent publications, however, suggest that beyond the interaction of Nod signals with a putative receptor, certain structural features of Nod factors are involved in controlling the concentration of the signals during their uptake by the root tissue.
    Type of Medium: Electronic Resource
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