Publication Date:
1987-12-04
Description:
A specific inhibitor of the neuraminidase of the protozoan parasite Trypanosoma cruzi was isolated recently and named cruzin. It is now shown that cruzin is similar to high-density lipoprotein by amino acid homology, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by immunoblot analysis, and by isoelectric focusing. Cruzin purified by ion exchange chromatography and high-density lipoprotein isolated by density gradient ultracentrifugation inhibited Trypanosoma cruzi neuraminidase to the same extent. Cruzin or high-density lipoprotein restores to normal the decreased multiplication rate of Trypanosoma cruzi epimastigotes grown in a medium depleted of lipoproteins, suggesting that it may be important for survival of the parasite in nature.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Prioli, R P -- Ordovas, J M -- Rosenberg, I -- Schaefer, E J -- Pereira, M E -- AI 07380/AI/NIAID NIH HHS/ -- AI 18102/AI/NIAID NIH HHS/ -- HL 35243/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1987 Dec 4;238(4832):1417-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Geographic Medicine and Infectious Diseases, New England Medical Center Hospitals, Inc., Boston, MA 02111.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3120314" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
*Anti-Infective Agents
;
Apolipoprotein A-I
;
Apolipoproteins A/genetics/immunology
;
Blood Proteins/immunology/pharmacology/*physiology
;
Isoelectric Focusing
;
Lipoproteins, HDL/*physiology
;
Neuraminidase/antagonists & inhibitors
;
Trypanosoma cruzi/*enzymology/growth & development
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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