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  • Nicotine  (2)
  • Acyl lipid formation  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Inhibition of carotenoid synthesis in Myxococcus fulvus (Myxobacterales) (1974)
    Springer
    Archives of microbiology 97 (1974), S. 217-226 
    Details
    ISSN: 1432-072X
    Keywords: Myxobacteria ; Carotenoid Glucosides ; Carotenoid Synthesis ; Nicotine ; Diphenylamine ; Herbicides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. The inhibitory effects of CPTA, nicotine, DPA, and San 6706 on carotenogenesis in Myxococcus fulvus were investigated. 2. The effects of CPTA, D-nicotine, and L-nicotine were very similar. The action of the drugs wasadditive. The cyclization was inhibited at low doses, the introduction of the hydroxyl group at C-1′ at higher doses. Lycopene accumulated at high drug concentration. The mode of action of the inhibitors is discussed. 3. In a carotenoid mutant of M. fulvus a stimulation of the “7,8-dehydrogenase” by CPTA was observed. 4. The specific carotenoid content of bacteria was increased by DPA due to an enhanced formation of phytoene. At low doses of DPA small amounts of an intermediate carotenoid glucoside ester, a 7,8-dihydro derivative, were detected. 5. DPA was taken up by the plasma membrane. Quantitative removal of DPA by washing was not possible. 6. San 6706 specifically and reversibly blocked the desaturation of phytoene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403061
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  • 2
    Electronic Resource
    Electronic Resource
    Carotenoids of Rhizobia (1978)
    Springer
    Archives of microbiology 119 (1978), S. 71-74 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium lupini ; Carotenoids ; Nicotine ; CPTA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract With increasing concentrations in the growth medium of the cyclization inhibitors nicotine or 2-(4-chlorophenylthio)-triethylamine hydrochloride (CPTA) the previously identified bicyclic carotenoids of Rhizobium lupini (2,3,2′,3′-tetrahydroxy-β,β-caroten-4-one and 2,3,2′,3′-tetrahydroxy-β,β-carotene) were successively replaced by hitherto unknown monocyclic carotenoids. By application of mass and nuclear magnetic resonance spectroscopy 3 carotenoids were identified as 2,3-trans-dihydroxy-β,ψ-caroten-4-one, 2,3-trans-dihydroxy-β,ψ-carotene, and 3-hydroxy-β,ψ-caroten-4-one. A further compound was tentatively established as (2- or 3-)monohydroxy-β,ψ-carotene. It was found that other inhibitors such as diphenylamine or 4-chloro-5-(dimethylamino)-2-α,α,α(trifluoro-m-tolyl)-3-(2H)-pyridazinone (San 6706) did not affect the pigment pattern. The results are discussed in relation to carotenoid biosynthesis in Rhizobium lupini.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407930
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  • 3
    Electronic Resource
    Electronic Resource
    Prenyl lipid and fatty-acid synthesis in isolatedAcetabularia chloroplasts (1990)
    Springer
    Planta 181 (1990), S. 229-233 
    Details
    ISSN: 1432-2048
    Keywords: Acetabularia ; Acyl lipid formation ; Chloroplast (fatty-acid synthesis) ; Fatty acid (synthesis) ; Prenyl lipid formation ; Mevalonate pathway
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gentle procedure allowed the isolation of intact and highly active chloroplasts from the unicellular green algaAcetabularia mediterranea. These chloroplasts incorporated carbon from NaH14CO3 into fatty acids and prenyl lipids at a rate of about 20–50 nmol carbon· (mg chlorophyll)−1·h−1. Most of the fatty acids formed in vitro were esterified in galactolipids. The main prenyl lipids synthesized were the chlorophyll side chain, intermediates of the carotenogenic path, α-and β-carotene, as well as lutein. Large amounts of [1-14C]acetate were incorporated, but exclusively into fatty acids.Isopentenyl diphosphate was a good substrate for prenyl-lipid formation in hypotonically treated chloroplasts. The envelope of intact chloroplasts, however, was impermeable to this compound. Intermediates of the mevalonate pathway were not accepted as precursors under conditions whereisopentenyl diphosphate was well incorporated. The results show that the lipid biosynthetic pathways in the plastids ofAcetabularia, a member of the ancient family of Dasycladaceae, are very similar to those in higher-plant plastids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02411543
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