Publication Date:
2007-03-24
Description:
Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ishii, Nobuyoshi -- Nakahigashi, Kenji -- Baba, Tomoya -- Robert, Martin -- Soga, Tomoyoshi -- Kanai, Akio -- Hirasawa, Takashi -- Naba, Miki -- Hirai, Kenta -- Hoque, Aminul -- Ho, Pei Yee -- Kakazu, Yuji -- Sugawara, Kaori -- Igarashi, Saori -- Harada, Satoshi -- Masuda, Takeshi -- Sugiyama, Naoyuki -- Togashi, Takashi -- Hasegawa, Miki -- Takai, Yuki -- Yugi, Katsuyuki -- Arakawa, Kazuharu -- Iwata, Nayuta -- Toya, Yoshihiro -- Nakayama, Yoichi -- Nishioka, Takaaki -- Shimizu, Kazuyuki -- Mori, Hirotada -- Tomita, Masaru -- New York, N.Y. -- Science. 2007 Apr 27;316(5824):593-7. Epub 2007 Mar 22.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17379776" target="_blank"〉PubMed〈/a〉
Keywords:
Chromatography, Liquid
;
Computational Biology
;
Electrophoresis, Capillary
;
Electrophoresis, Gel, Two-Dimensional
;
Enzyme Induction
;
Enzyme Repression
;
Enzymes/genetics/metabolism
;
Escherichia coli/enzymology/*genetics/growth & development/*metabolism
;
Escherichia coli Proteins/genetics/*metabolism
;
Gene Expression
;
*Genes, Bacterial
;
Mass Spectrometry
;
*Metabolic Networks and Pathways/genetics
;
Mutation
;
Oligonucleotide Array Sequence Analysis
;
Proteome
;
RNA, Messenger/genetics/metabolism
;
Systems Biology/*methods
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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