ISSN:
1432-072X
Keywords:
pppApp-synthesizing activity
;
ppGpp and pppGpp-purine nucleotide pyrophosphotransferase
;
Actinomycetes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The occurrence of adenosine 5′-triphosphate-3′-diphosphate-synthesizing activity was detected in five strains of actinomycetes; Streptomyces morookaensis, Streptomyces aspergilloides, Streptomyces hachijoensis, Actinomyces violascens and Streptoverticillium septatum, out of 825 strains of actinomycetes, bacteria, fungi and imperfecti. Purine nucleotide pyrophosphotransferase were extracellularly excreted associating with the cell growth, and were purified partially or to apparent homogeniety from the culture filtrate. The enzymes are a monomeric protein with a molecular weight of 18000–26000 and synthesize adenosine, guanosine and inosine 5′-phosphate (mono, di or tri)-3′-diphosphate such as pApp, ppApp, pppApp, pGpp, ppGpp, pppGpp and pppIpp by transferring a pyrophosphoryl group from the 5′-position of ATP, dATP and pppApp to the 3′-position of purine nucleotides in the presence of a divalent cation and in alkaline state.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00446837
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