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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 48 (1988), S. 109-115 
    ISSN: 1570-7458
    Keywords: Acyrthosiphon pisum ; pea aphid ; allelochemicals ; isoxazolinone compounds ; Lathyrus toxin ; symbionts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Le rôle de cinq acides aminés non protéiques et de deux nitriles, composés qui sont des métabolites secondaires majeurs dans les plantules de Pisum sativum L., Lathyrus odoratus L., ou L. tingitanus L., a été évalué séparément dans la phagostimulation et la survie du puceron du pois, Acyrthosiphon pisum (Harris) (Homoptera: Aphididae). Le β-(isoxazolin-5-one-2-yl)-alanine (I), le 2-cyanoethyl-isoxazolin-5-one (VIII) et L-homoarginine ont été hautement phagostimulants, la lathyrine et le fumarate de β-aminopropionitrile (BAPN) ont eu un effet répulsif, tandis que le 2-(3-amino-3-carboxypropyl)-isoxazolin-5-one (VI) et l'isowillardiine (II) n'eurent aucun effet répulsif ni phagostimulant. Les composés homoarginine, I, VI et VIII ont été bénéfiques à la survie, contrairement aux composés II et lathyrine qui n'eurent aucun effet sur la survie de l'insecte.
    Notes: Abstract Five nonprotein amino acids and two nitriles, which are major secondary metabolites in the seedlings of Pisum sativum L., Lathyrus odoratus L., or Lathyrus tingitanus L., were individually tested for their role in the phagostimulation and survival of the pea aphid, Acyrthosiphon pisum (Harris) (Homoptera: Aphididae). β-(isoxazolin-5-one-2-yl)-alanine (I), 2-cyanoethyl-isoxazolin-5-one (VIII), and L-homoarginine were highly phagostimulatory, lathyrine and β-aminopropionitrile fumarate (BAPN) had a deterrent effect, and 2-(3-amino-3-carboxypropyl)-isoxazolin-5-one (VI) and isowillardiine (II) were neither phagostimulatory nor inhibitory. Homoarginine, I, VI, and VIII were beneficial for survival; whereas II and lathyrine had no effect on survival.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 26 (1990), S. 30-39 
    ISSN: 1040-452X
    Keywords: Acrosome reaction ; Electron microscopy ; Acrosomal stain ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Three staining procedures to detect sperm acrosome integrity were compared via electron microscopy. Stains were applied to epididymal, freshly ejaculated, in vivo capacitated, and sonicated sperm cells in addition to spermatozoa displaying sequentially removed plasma and outer and inner acrosomal membranes. Sequential membrane removal procedures resulted in removal of plasma membranes from 73% of all sperm cells, removal of plasma and outer acrosomal membranes from 74% of all sperm cells, and removal of plasma and outer and inner acrosomal membranes from 87% of all sperm cells as determined by electron microscopy. Live/dead staining results were not statistically different from subjective microscopic motility evaluations (P 〈 0.005) for epiddymal, sonicated, freshly ejaculated, and in vivo capacitated sperm samples. All three stains assessed were similarly capable of detecting the acrosome status of freshly ejaculated and of sonicated spermatozoa compared to data obtained by electron microscopy (P = 0.010). However, only the Bryan-Akruk stain afforded data that were closely correlated with data obtained via electron microscopy for all sperm types assessed; the latter included in vivo capacitated spermatozoa and sperm cells rendered free of plasma membranes. Results confirmed an earlier report by successfully effecting sequential removal of rabbit acrosomal membrances and documented use of the Bryan-Akruk acrosomal stain for evaluation of sperm cell popualtions for fertilizing ability. These findings should prove useful in further investigations of mechanisms involved in achievement of fertilizing ability by rabbit spermatozoa.
    Additional Material: 9 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 13 (1986), S. 317-325 
    ISSN: 0148-7280
    Keywords: inner-acrosomal membrane ; antiserum ; FITC ; immunofluorescence ; sperm ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The acrosome-reacted spermatozoa interact with the zona pellucida through their limiting inner acrosomal membrane (IAM). The antigenic properties of IAM were determined using the antibody to IAM raised in a male guinea pig. The antisera was incubated with the acetone powder of rabbit lung, liver, kidney, heart, muscle, and blood cells. The unabsorbed antibodies specifically interacted with antigens on the IAM as determined by the immunofluorescence technique.
    Additional Material: 8 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 4 (1987), S. 161-168 
    ISSN: 0739-4462
    Keywords: aphids ; nucleotides ; sucrose ; symbionts ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Protein, nucleic acids, and nucleotide syntheses were studied in pea aphids, Acyrthosiphon pisum (Harris), by feeding them labeled 14C-amino acids and [5-3H]-orotic acid in sucrose. It was demonstrated that in the absence of dietary essential amino acids, aphids were capable of synthesizing nucleic acids, nucleotides, and proteins when provided with a single dietary amino acid in sucrose. It is suggested that other required amino acids were possibly supplied by the symbionts present in the pea aphid and/or were obtained from the amino acid pool in the hemolymph or glucose, one of the end products of sucrose digestion. Of the various amino acids tested, synthesis of measurable amounts of protein or other compounds occurred when alanine, aspartic acid, glutamic acid, glycine, proline, or serine were provided, but no synthesis occurred with cysteine.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
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