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  • 1
    Electronic Resource
    Electronic Resource
    Regulation of isocitrate lyase in a mutant of Rhodopseudomonas capsulata adapted to growth on acetate (1979)
    Springer
    Archives of microbiology 120 (1979), S. 43-46 
    Details
    ISSN: 1432-072X
    Keywords: Isocitrate lyase ; Regulation ; Acetate metabolism ; Rhodopseudomonas capsulata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Studies on acetate utilization by Rhodopseudomonas capsulata strain St. Louis indicated that the wild type grew poorly on acetate and made little if any of the glyoxylate cycle enzyme isocitrate lyase. A spontaneous mutant, Ac-l, capable of vigorous and immediate growth on acetate and exhibiting high levels of isocitrate lyase activity, was isolated in the course of those studies. Isocitrate lyase was not formed when the mutant was grown on malate. Addition of malate to cultures of Ac-l growing on acetate resulted in loss of the enzyme by dilution through growth. Starvation of acetate-grown Ac-l for acetate resulted in a rapid and complete loss of isocitrate lyase activity which was shown to be energy dependent. Readdition of acetate to a starved culture previously grown on acetate resulted in a rapid recovery of enzyme activity. The recovery required energy and was sensitive to chloramphenicol inhibition at any time during the recovery phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413271
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  • 2
    Electronic Resource
    Electronic Resource
    Photoheterotrophic utilization of acetate by the wild type and an acetate-adapted mutant of Rhodopseudomonas capsulata (1979)
    Springer
    Archives of microbiology 120 (1979), S. 39-42 
    Details
    ISSN: 1432-072X
    Keywords: Acetate metabolism ; Rhodopseudomonas capsulata ; Isocitrate lyase ; Rhodospirillaceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhodopseudomonas capsulata strain St. Louis can grow anaerobically in the light-with acetate as the carbon source. The organism is sensitive to acetate, however, initial concentrations exceeding 25 mM resulting in an extensive growth lag. Bicarbonate is not required for growth of this strain on acetate, but addition of bicarbonate shortens the lag phase in media with high initial acetate concentration. A spontaneous mutant which exhibited a minimal lag phase and rapid growth rates on acetate media was derived from strain St. Louis. This mutant possessed elevated levels of the glyoxylate cycle enzyme, isocitrate lyase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413270
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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