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  • 1
    ISSN: 1573-5036
    Keywords: Acer saccharum ; forest decline ; soil fertility ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of an artificially induced drought was investigated in an 80-year-old sugar maple (Acer saccharum Marsh.) stand in southern Quebec. We hypothesized that leaf peroxidase activity would increase, and that leaf nutrient deficiencies typical of declining trees (K, Mg) would develop during the year following the imposition of the drought. An impermeable tarpaulin was placed on the ground at the base of the trees during the summer of 1991. Foliage and soils were sampled periodically during the summers of 1991 and 1992. The treated trees showed decreased leaf water potential and increased peroxidase activity over most of the 1991 growing season. Diameter growth was significantly reduced in 1992. Soil NO 3 - and K+ were elevated while Mn2+ was reduced under the tarpaulin treatment at various times during the 1991 growing season. Soil NO 3 - , NH 4 + , K+ and Mg2+ were elevated in the treated plots at various times during the 1992 growing season even though there was a lack of effect on foliar nutrient concentrations over the same period. Drought conditions may have reduced water and nutrient uptake without significantly affecting leaf nutrient status.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5036
    Keywords: Acer saccharum ; nutrient status ; plant stress ; soil frost ; peroxidases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effect of removal of snow cover in winter was investigated in an 80-year-old sugar maple (Acer saccharum Marsh.) stand in southern Quebec. We hypothesized that winter soil frost would induce some of the decline symptoms observed in sugar maple stands in southern Quebec in the early 1980's. Snow was continuously removed from around trees for a one week (partial removal) or for a four-month period (complete removal) during the 1990–1991 winter. Foliage and soils were sampled periodically during the summer of 1991. The complete snow removal treated trees showed decreased leaf water potential and increased peroxidase activity over most of the growing season. Foliar Ca was reduced in both snow removal treatments early in the growing season while foliar N was reduced in the complete snow removal trees late in the growing season. Soil NO 3 − and K+ were elevated in both snow removal treatments at various times throughout the growing season. Prolonged soil frost in a sugar maple stand can induce lower leaf water potential, higher leaf peroxidase activity and early leaf senescence during the following growing season. Soil frost may have reduced nutrient uptake without affecting significantly the leaf nutrient status.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electrophoresis 4 (1983), S. 164-165 
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Nonspecific staining of electrofocused protein bands was found with the peroxidase product of Hanker-Yates reagent (a mixture of p-phenylenediamine and pyrocatechol). No difficulty was encountered with diaminobenzidine as peroxidase substrate.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electrophoresis 4 (1983), S. 367-367 
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Porous supports for electroblot transfers of human tumor associated antigens from agarose slab gels allow easier slab gel handling and minimize possible distortion of the protein transfers.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electrophoresis 4 (1983), S. 368-369 
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The use of peroxidase-conjugated antibodies for detecting specific tumor associated antigens bound to nitrocellulose membranes is being increasingly used in our laboratory. This method offers many advantages. However, we have observed several problems which can cause confusing results and possible misinterpretations. Some methods of eliminating artifacts are described as well as the use of glutaraldehyde vapor fixation as a means to increase sensitivity.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Two-dimensional (crossed) immunoelectrophoresis using a two-chamber csting cassette and a high gel strenght, low gelling temperature agarose mixture, can now be done with preformed thin antibody-containing second dimension slab gels in a procedure that is less skill-dependent and less injurious to heat-labile macromolecules.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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