ISSN:
1573-4994
Keywords:
Absorption
;
fluorescence probe
;
dipole moment
;
electro-optical method
;
dielectric constant
;
erythrocyte ghosts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Physics
Notes:
Abstract Electrooptical absorption and emission methods were used to measure the dipole moments of 1-phenylnaphthylamine (1-AN) and 1,8-anilinonaphthalene sulfonate (1,8-ANS) fluorescence probes in the ground and excited states. The measurements were performed in cyclohexane (1-AN) and dioxane (1-AN and 1,8-ANS). Our results show that the charge distribution in 1-AN differs substantially from that in 1,8-ANS. The spectral dependence of electrooptical coefficients in the case of 1,8-ANS indicates that the absorption band is a superposition of several (at least two) electronic transitions. We found that the electronic spectra of 1-AN in erythrocyte ghosts are inhomogeneously broadened. Due to inhomogeneous broadening it is possible to excite selectively molecules located at different sites. Dielectric interactions (given by the dielectric constant) were investigated from the position of the maximum of the fluorescence quantum spectra of 1-AN. The dielectric constant of human erythrocyte ghosts varies from about 6.7 ± 0.8 to about 17.6 ± 3.5, depending on the excitation frequency and, consequently, on the location of the probe.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1022598430510
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