ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • AbbreviationsNAA: Naphthaleneacetic acid  (1)
  • Gynogenesis  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Gene transfer into isolated and cultured tobacco zygotes by a specially designed device for electroporation (2000)
    Springer
    Plant cell reports 19 (2000), S. 1184-1187 
    Details
    ISSN: 1432-203X
    Keywords: Keywords Zygote ; In vitro culture ; Electroporation ; Transformation ; Tobacco ; AbbreviationsNAA: Naphthaleneacetic acid ; ¶BA: 6-Benzyladenine ; GUS: β-Glucuronidase ; ¶GFP: Green fluorescent protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have established a technique for isolating, culturing and transforming tobacco zygotes. Zygotes were isolated by microdissection or enzymatic maceration from fertilized embryo sacs. Viable zygotes cocultured with mesophyll protoplasts underwent first division after 3 days of culture. Zygotes isolated by microdissection underwent a higher frequency of first division (61.2%) than those isolated by enzymatic maceration (30.5%). Globular embryos were formed only from microdissected zygotes, at a frequency of 8.7% after 1–2 weeks in culture. An efficient millicell device for the electroporation of DNA into zygotes was established. The electroporated zygotes divided in vitro at a frequency of 54.6% and developed into proembryos. Introduced GFP gene constructs showed transient expression in about 2.6% of the electroporated tobacco zygotes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000249
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    In vitro induction of haploid plants from unpollinated ovaries and ovules (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 97-104 
    Details
    ISSN: 1432-2242
    Keywords: Haploid induction ; Ovary culture ; Ovule culture ; Gynogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A recent advance in plant tissue culture and experimental embryology is the successful induction of haploid plants by the culture of unpollinated ovaries or ovules. It means that not only the microspore but also the megaspore or female gametophyte of angiosperms can be triggered in vitro to sporophytic development, thus opening a new way to genetical research and haploid breeding. Since data so far accumulated are attractive, though not so rich, this paper tends to offer a preliminary review on this problem, covering the historical description, culture experiments, embryological observations, cytological and genetical studies and a brief discussion on its perspectives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303687
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...