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  • Incremental method of gradient elution  (2)
  • Abbreviations: BAP: 6-benzyladenine phosphate; BPMV: bean pod mottle virus; CP-P: coat protein-precursor; CTAB: hexadecyltrimethylammonium bromide; DAS-ELISA: double antibody sandwich-enzyme-linked immunosorbent assay; IBA: indole-butyric acid; kbp: kilobase pairs; MES: 2-(N-Morpholino)ethanesulfonic acid; NOS: nopaline synthase; NPTII: neomycin phosphotransferase II; NTP: nucleoside triphosphate; PBS: phosphate-buffered saline; PCR: polymerase chain reaction; PVP: polyvinyl pyrrolidone; VPg: viral genome-linked protein.  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene (1996)
    Springer
    Plant cell reports 15 (1996), S. 746-750 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: BAP: 6-benzyladenine phosphate; BPMV: bean pod mottle virus; CP-P: coat protein-precursor; CTAB: hexadecyltrimethylammonium bromide; DAS-ELISA: double antibody sandwich-enzyme-linked immunosorbent assay; IBA: indole-butyric acid; kbp: kilobase pairs; MES: 2-(N-Morpholino)ethanesulfonic acid; NOS: nopaline synthase; NPTII: neomycin phosphotransferase II; NTP: nucleoside triphosphate; PBS: phosphate-buffered saline; PCR: polymerase chain reaction; PVP: polyvinyl pyrrolidone; VPg: viral genome-linked protein.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Soybean (Glycine max. Merrill. cv. Fayette) cotyledonary nodes were transformed with bean pod mottle virus (BPMV) coat protein precursor (CP-P) gene via Agrobacterium-mediated transformation. The transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. Southern blot hybridization verified the integration of the BPMV CP-P gene. Inheritance and expression of this gene in R1 plants were also demonstrated. About 30% of R2 plants derived from one transgenic line showed complete resistance to BPMV infection, as assessed by symptomatology and ELISA, suggesting that homozygous, but not hemizygous, plants exhibit the resistant phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050112
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  • 2
    Electronic Resource
    Electronic Resource
    The varigrad and similar apparatus in gradient liquid chromatography: A systematic computational approach. Part 2: Applications (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 575-582 
    Details
    ISSN: 0935-6304
    Keywords: Numerical examples ; Constant volume Varigrad ; Hydrostatic Varigrad ; Incremental method of gradient elution ; Modified incremental method ; Non-homogeneous Varigrad ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Part 2 deals with the application of the equations derived in Part 1 to the systematic calculations of the constant volume and hydrostatic Varigrads. Examples are given which illustrate the advantage of using the modified incremental method of gradient elution. The non-homogeneous Varigrad, where mixing chambers may vary in volume, is also treated.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240031106
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  • 3
    Electronic Resource
    Electronic Resource
    The Varigrad and similar apparatus in gradient liquid chromatography: A systematic computational approach. Part 1: Theoretical development (1980)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 507-515 
    Details
    ISSN: 0935-6304
    Keywords: Constant volume Varigrad ; Hydrostatic Varigrad ; Incremental method of gradient elution ; Modified incremental method, advantages of Poisson distribution ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Different methods for producing a pre-assigned gradient in liquid chromatography are presented. A systematic approach to the calculations involved is given. The derived equations are mostly Poisson and Poisson summation distributions which are tabulated in the literature. The incremental method of gradient elution developed by Scott has been modified. In the modified apparatus two mixing chambers are used instead of one. This leads to an appreciable decrease in the number of reservoirs needed for the same precision in fitting a desired gradient. The application of the derived equations, together with other Varigrad modifications, will be given in Part 2 of this paper (to be published shortly in this Journal).
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240031005
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