ISSN:
1573-0972
Keywords:
Alkaline phosphatase
;
fungi
;
molecular biology
;
Neurospora crassa
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Alkaline phosphatase, excreted by Neurospora crassa preg c and purified to apparent homogeneity by 7.5% PAGE, did not show DNAase activity and removed the terminal 5′-phosphate group from plasmid Bluescript M13+ linearized with EcoRI. The preg c strain may therefore replace other sources of alkaline phosphatase for use in dephosphorylating linearized plasmidial DNA in cloning experiments.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00286362
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