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  • 1
    Electronic Resource
    Electronic Resource
    Impact of acute temperature transition on enzyme activity levels, oxygen consumption, and exogenous fuel utilization in sea raven (Hemitripterus americanus) hearts (1990)
    Springer
    Journal of comparative physiology 160 (1990), S. 511-518 
    Details
    ISSN: 1432-136X
    Keywords: ATP ; Fatty acid metabolism ; Fish ; Glycolysis ; Heart ; Oxygen consumption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The impact of an acute temperature transition between 5 °C and 15 °C on energy metabolism and performance of sea raven (Hemitripterus americanus) heart was assessed. Maximal in vitro activity of hexokinase was 1.2 and 3.7 μmol · min-1 · g-1 at 5 °C and 15 °C, respectively. Carnitine palmitoyl transferase and carnitine palmitoleoyl transferase activities were 0.07 μmol · min-1 · g-1 at 15 °C and declined substantially at 5 °C. Oxygen consumption and power output of perfused isolated hearts offered glucose alone as a metabolic fuel decreased significantly between 15 °C and 5 °C. When palmitoleate was included in the perfusion medium, oxygen consumption and power development remained constant between 15 °C and 5 °C, suggesting that glucose alone was not an adequate metabolic fuel at low temperature. However, maximal in vitro activity of HK implied that the catalytic potential at this locus was quite capable of meeting demands of carbon flow, while the maximal in vitro activity of the carnitine acyl CoA transferases implied that fatty acid metabolism should be greatly compromised at low temperatures. In an effort to resolve the contradiction, hearts were perfused with medium containing 14C-glucose or 14C-palmitate. Rates of 14CO2 production from labelled metabolic fuels could account for only about 2% of the oxygen consumption rates. Most of the label from 14C-glucose was incorporated into the glycogen and lipid fractions and label from 14C-palmitate was incorporated into the lipid fraction. The net incorporation rates of label into intracellular pools were temperature insensitive over the range 5–15 °C. The incorporation of exogenous glucose into the lipid fraction suggests that activity of the entire glycolytic pathway was maintained over the temperature range. Thus, the relatively low rate of oxygen consumption of hearts perfused with glucose alone as an exogenous substrate cannot be attributed to a limitation of glucose catabolism. The alternative explanation is that the presence of fatty acids induces an increase in oxygen consumption, especially at 5 °C. It is speculated that this is due to alterations in Ca2+ balance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00258978
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    Paper (German National Licenses)
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  • 2
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    Improved limit on the ^{225} Ra electric dipole moment (2016)
    American Physical Society (APS)
    Details
    Publication Date: 2016-08-04
    Description: Author(s): Michael Bishof, Richard H. Parker, Kevin G. Bailey, John P. Greene, Roy J. Holt, Mukut R. Kalita, Wolfgang Korsch, Nathan D. Lemke, Zheng-Tian Lu, Peter Mueller, Thomas P. O'Connor, Jaideep T. Singh, and Matthew R. Dietrich Background : Octupole-deformed nuclei, such as that of Ra 225 , are expected to amplify observable atomic electric dipole moments (EDMs) that arise from time-reversal and parity-violating interactions in the nuclear medium. In 2015 we reported the first “proof-of-principle” measurement of the Ra 225 ato… [Phys. Rev. C 94, 025501] Published Wed Aug 03, 2016
    Keywords: Electroweak Interaction, Symmetries
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
    Published by American Physical Society (APS)
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