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  • Articles  (17)
  • Polymer and Materials Science  (11)
  • Engineering General  (3)
  • nucleotide sequence  (3)
  • ASTRONOMY
  • 1
    Electronic Resource
    Electronic Resource
    Characterisation of aminoglycoside acetyltransferase-encoding genes of neomycin-producing Micromonospora chalcea and Streptomyces fradiae
    Amsterdam : Elsevier
    Gene 101 (1991), S. 143-148 
    Details
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; antibiotic resistance ; homologies ; nucleotide sequence ; promoter mapping
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(91)90237-6
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  • 2
    Electronic Resource
    Electronic Resource
    Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae
    Amsterdam : Elsevier
    Gene 25 (1983), S. 179-187 
    Details
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; aminocyclitol-modifying enzyme ; cross-species selective marker ; nucleotide sequence
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0378-1119(83)90223-8
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  • 3
    Electronic Resource
    Electronic Resource
    Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae
    Amsterdam : Elsevier
    Gene 25 (1983), S. 179-188 
    Details
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; [abr] DTT; dithiothreitol ; [abr] EtBr; ethidium bromide ; [abr] HPH; hygromycin B phosphotransferase protein ; [abr] ORF; open reading frame ; [abr] SD; ribosomal binding site (Shine and Dalgarno,1974) ; [abr] SDS; sodium docecyl sulfate ; [abr] TBE; see MATERIALS AND METHODS,sectiond ; [abr] YEPD; YEPG YM media,ee MATERIALS METHODS,sectionb ; [abr] [ ]; indicates plasmid-carrier state ; [abr] amp; ampicillin ; [abr] bp; base pairs ; [abr] hph; hygromycin B phosphotransferase gene ; [abr] hyg; hygromycin B ; [abr] hyg^r; hygromycin B-resistant ; [abr] hyg^s; hygromycin B-sensitive ; [abr] kb; kilobase pairs ; [abr] tet; tetracycline ; aminocyclitol-modifying enzyme ; cross-species selective marker ; nucleotide sequence
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0378-1119(83)90223-8
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  • 4
    Electronic Resource
    Electronic Resource
    Finite element bending analysis of multilayer plates (1974)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 8 (1974), S. 215-225 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The development of a general quadratic multilayer plate element is presented for the analysis of arbitrarily layered curved plates. In the formulation, each layer of the multilayer plate can have different orthotropic properties and can deform locally. Examples of bending problems are presented which demonstrate the applicability of the formulation.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620080203
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  • 5
    Electronic Resource
    Electronic Resource
    The shear deformation of profiled metal sheeting (1978)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 12 (1978), S. 1507-1541 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In the stressed-skin analysis of metal structures it is vital to have a simple yet accurate treatment of the shear deformation of profiled metal sheeting. A suitable theory is developed in this paper for the two important cases of sheeting fastened to the supporting structure through every and alternate troughs of the corrugations. Various practical factors are taken into account. The results of an extensive series of numerical investigations are presented as a simple equation and a set of tables.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620121005
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  • 6
    Electronic Resource
    Electronic Resource
    A new formulation of the plastic design problem for plane frames (1972)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 5 (1972), S. 185-192 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Using a parametrie representation, it was proved in 19591 that the static and kinematic approaches to the plastic collapse problem are dual linear programming problems. In this paper, the generalized parameters used previously are given physical meaning and shown to be readily programmed for computer application. One important advantage of the method is that the equations can be written in general terms such that no assumptions regarding the structural from need to be built into the program. The application is extended to the more general problem of automatic plastic design and it is shown that the inclusion of a serviceability constraint (e.g. no plastic hinges at a given load factor) is trivial in this formulation. The difficult problem of several loading cases is shown to be readily, treated by designing for a given load factor for shakedown rather than plastic collapse. Thought this latter facility, by its dependence on elastic behavior, requires iteration of the stiffness of the structure, repaid convergence appears to be generally obtained. Finally a method is suggested whereby an adequate allowance for frame instability may be made without destroying the linearity of the equations. Thought the equations, as developed, have application to steel framed structures in mind, the extension to the limit design of reinforced concrete structures appears to present no addition complications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620050205
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  • 7
    Electronic Resource
    Electronic Resource
    The bone-titanium interface in vitro (1990)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 24 (1990), S. 1289-1306 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Commercially pure 5-mm-diameter titanium (cpTi) discs received droplet inoculations of cells derived from rat bone marrow and were maintained in supplemented culture medium for 2-3 weeks. The cells and extracellular matrix (ECM) were processed for observation by light (LM), scanning (SEM), and transmission electron (TEM) microscopy. The latter was achieved by freeze-fracturing the solid metal from the resin-embedded tissue using a method which preserved the interface. Surface staining of whole discs revealed cells separated from the metal substratum by areas of ECM which stained positively using von Kossa's method to identify mineralization. At SEM, the ECM comprised dense interwoven collagen fiber networks which were partially obscured by globular masses (GMs). Individual GMs were associated with collagen fibers, especially at fiber intersections. EDAX line scan analysis confirmed the presence of Ca and P in these areas which were assumed to be spheritic foci of calcification since the Ca and P peaks diminished in areas which demonstrated only collagen fibers or the underlying cpTi. TEM examination confirmed the presence of globular mineralization and also revealed the presence of an interfacial zone between the metal substratum and the mineralized ECM elaborated by osteoblasts during the culture period. The interfacial zone comprised two layers, a bonding zone containing few collagen fragments and a ruthenium red positive layer containing more densely packed collagen fibers. We believe that this is the first report of both the formation of bonelike tissue on solid titanium substrata in vitro and demonstration of an interface which bears close morphological similarities to that known to develop in vivo.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820241003
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  • 8
    Electronic Resource
    Electronic Resource
    Behavior of primary bone cells on characterized polystyrene surfaces (1993)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 27 (1993), S. 851-859 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Primary bone cells, isolated from the periosteally stripped calvariae of neonate rats, were cultured on 60Co γ-irradiation-sterilized bacteriologic-grade polystyrene that had been either surface treated with concentrated sulfuric acid or received further γ-irradiation treatments facilitated cell colonization of the polystyrene compared to those surfaces not treated in the laboratory. x-Ray photoelectron spectroscopy (XPS) showed that the two treatments introduced different chemical groups onto the polymer surface and that cell adhesion was related to γ-irradiation in a dose-dependent manner. These results show that simple biologic assays, such as cell colonization, are not able to distinguish between differences in surface chemistry demonstrated by such a routinely employed surface analysis technique. Thus, there is a need to develop more sensitive biologic assays that provide functional information of a precision that can be correlated with subtle changes in substratum surface chemistry. Further, we argue that because cells isolated by tissue digestion using proteolytic enzymes respond more readily to changes in the surface chemistry of the substratum they colonize, compared to explanted cells; biologic assays designed for biomaterials testing must take into account changes effected in cell adhesion behavior by isolation procedures. © 1993 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820270703
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  • 9
    Electronic Resource
    Electronic Resource
    Interfacial behavior of PEO/PBT copolymers (polyactive®) in a calvarial system: An in vitro study (1994)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 28 (1994), S. 269-277 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Polyactive®, a polyethylene oxide/ polybutylene terephthalate (PEO/PBT) copolymer, has been reported to display bone-bonding behavior. Although a detailed description of the in vivo bone/ Polyactive® interface is available, the underlying bone-bonding mechanism is still largelyunknown. In this in vitro study, a calvarial envelope method has been adopted to reproduce the in vivo bone-bonding phenomenon and subsequently to obtain information on the biological effect of varying PEO/PBT segment ratios. The following PEO/PBT ratios were examined: 70/30, 60/40, 55/45, 40/60, and 30/70. Light microscopy (LM) and scanning (SEM), transmission (TEM), and backscatter electron microscopy (BSE), as well as X-ray microanalysis (XRMA), were employed. Within the period of analysis (3 weeks), an intimate contact between mineralized deposition and the 70/30, 60/40, and, to a lesser extent, the 55/45 surface was observed. Calcified areas developed within the surface of these PEO/BPT proportions during the culture period. Needle-shaped crystals from the mineralized tissue compartment and from calcified areas within the materials surface were intermingled at the interface, providing a morphologic continuity. A cellular layer was interposed with the mineralization front and the noncalcified 40/60 and 30/70 substrates. Apparently, the percentage of PEO is important for calcification within the near surface of the polymer. This relation is such that the highter the PEO content in PEO/PBT ratios, the more rapid the calcification is considered. The occurrence of material calcification is considered to be largely responsible for the subsequent interfacial interactions. The calvarial envelope culture method allows not only reproduction of the in vivo bone/Polyactive® interface, but also a relatively rapid differentiation within the range of PEO/PBT ratios. It was therefore concluded that this in vitrosystem is suitable for further studies toward a better understanding of the bone/Polyactive® interfacial composition and the underlying mechanisms. © 1994 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820280218
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  • 10
    Electronic Resource
    Electronic Resource
    Initial bone matrix formation at the hydroxyapatite interface in vivo (1995)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 29 (1995), S. 89-99 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Dense, sintered, slip-cast hydroxyapatite rods were implanted transfemorally in young adult rats. The femora were excised after 2 and 4 weeks and, following fixation, either embedded in methyl methacrylate for light microscopy, decalcified and prepared for transmission electron microscopy, or freeze fractured in liquid nitrogen for scanning electron microscopic analysis. The latter was performed on the two tissue fragments that remained after freeze fracturing, from which the first contained the implants and the second comprised tissue that had been immediately adjacent to the hydroxyapatite rods. Undecalcified light microscopic sections revealed extensive bone tissue formation around and in contact with the hydroxyapatite rods. The initial bone matrix apposed to the implant surface, as demonstrated with scanning electron microscopy, was either composed of globular deposits or an organized network of collagen fibers. The deposits, which ranged in size from 0.1-1.1 μm, fused to form a cement-like matrix to which collagen fibers were attached. Degradation of the hydroxyapatite surface resulted in the presence of unidirectionally aligned crystallites, with which the newly formed bone matrix was closely associated. Ultrastructural analysis of the bone-hydroxyapatite interface with transmission electron microscopy revealed a 50-600-nm-wide collagen-free granular zone, comprising one or more 40-100-nm-thick electron-dense layer(s). These structural arrangements most probably partially represent the globular deposits and proteinaceous material adsorbed onto and partially in the degrading hydroxyapatite surface. Although the latter change in surface topography may have enhanced bonding of the cement-like matrix to the hydroxyapatite, the cause for this change in topography and the type of bond formed are, at present, unknown. © 1995 John Wiley & Sons, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820290113
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