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  • 1
    ISSN: 1570-7458
    Keywords: Ceutorhynchus assimilis ; oilseed rape ; host plant volatiles ; attractant ; olfactometer ; isothiocyanate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The responses of the cabbage seed weevil,Ceutorhynchus assimilis Payk. (Coleoptera: Curculionidae) to volatiles from oilseed rape (Brassica napus L.) were tested using a linear track olfactometer. Weevils were attracted towards the odour of rape during a short period before diapause and for most of their postdiapause life. Odours from both the flowering and green parts of the plant were attractive, but the odour of a non-host plant (Bellis perennis L.) was not. An entrainment extract of flowering rape volatiles in pentane was attractive, but significantly less so than the odour of flowering rape itself. Attraction was found to 3-butenyl and 4-pentenyl isothiocyanate (NCS), but not to 2-phenylethyl isothiocyanate. A mixture of the three NCSs was more attractive than the individual NCSs. However, the concentration required to produce a response was still relatively high. Isothiocyanates, along with other volatiles from rape, probably play a role in host plant recognition by the seed weevil.
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  • 2
    ISSN: 1570-7458
    Keywords: cabbage seed weevil ; Ceutorhynchus assimilis ; oilseed rape ; Brassica napus ; host-plant volatile ; olfactometer ; (Z)-3-hexen-1-ol ; methyl salicylate ; nitriles ; cyanides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cabbage seed weevil, Ceutorhynchus assimilis Payk. [syn. Ceutorhynchus obstrictus (Marsham)] (Coleoptera: Curculionidae), a crucifer-feeding insect, is a pest of oilseed rape (Brassica napus L.). It is known to be attracted by isothiocyanates, crucifer-specific volatiles that are metabolites of the glucosinolates. The responses of this insect to other electrophysiologically-active volatiles from rape were tested in a linear track olfactometer. Attraction was demonstrated to nitriles (phenylacetonitrile, 4-pentenenitrile and 5-hexenenitrile), which are also glucosinolate metabolites, and to volatiles emitted by a wider spectrum of plant families ((Z)-3-hexen-1-ol and methyl salicylate). Combination of an isothiocyanate mixture with phenylacetonitrile increased attraction, but there was no such increase when the isothiocyanate mixture was combined with methyl salicylate. A mixture of 23 volatiles, emulating an attractive air-entrainment extract of oilseed rape, was not significantly attractive, although a high proportion of weevils (60%) turned towards it. The potential of these volatiles for inclusion into an isothiocyanate-based monitoring system is discussed.
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  • 3
    ISSN: 1573-1561
    Keywords: Oviposition-deterring pheromone ; cabbage seed weevil ; Ceutorhynchus assimilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Behavioral bioassays have confirmed that the oviposition-deterring secretion of the cabbage seed weevil, Ceutorhynchus assimilis Payk., can be isolated from glass tubes marked by the weevil and from extracts of its dissected seventh urotergite. Analysis of the secretion by gas chromatography—mass spectrometry showed that it contained iso- and n-alkanes, dimethylalkanes, alkenes, fatty acids, 15-nonacosanonc, 15-nonacosanol, and cholesterol. The oviposition-deterring properties of the secretion are associated with a more polar traction, isolated by liquid chromatography front an extract of the seventh urotergite.
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  • 4
    ISSN: 1573-1561
    Keywords: Oviposition-deterring pheromone ; host marking pheromone ; marker ; electrophysiology ; contact chemoreception ; gustatory sensilla ; antenna ; behavior ; Ceutorhynchus assimilis ; Coleoptera ; Curculionidae ; Brassica napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Following oviposition into a pod of oilseed rape (Brassica napus), the female cabbage seed weevil (Ceutorhynchus assimilis) marks the pod with oviposition-deterring pheromone (ODP) by brushing it with her eighth abdominal tergite. On an unmarked pod, oviposition site selection was always accompanied by intensive antennation of the pod. Females approaching a freshly ODP-marked pod brought their antennae within 1 mm of the pod but usually did not antennate it before rejecting it for oviposition. Females with the clubs of their antennae amputated continued to discriminate pods from stems or petioles as oviposition sites but showed no behavioral response to ODP. Extracts of volatiles air-entrained from ovipositing weevils failed to inhibit oviposition. Air passed over a behaviorally active extract of ODP did not elicit a detectable electroantennogram response. By contrast, when presented as a gustatory stimulus to the sensilla chaetica of the antennal club, a behaviorally active extract of ODP from postdiapause, gravid females elicited a strong electrophysiological response. This response usually involved more than one cell and displayed a phasic–tonic time course over the recording period of 10 sec. Extract from prediapause (and hence sexually immature) females elicited neither behavioral nor electrophysiological (contact) responses. Thus the ODP of the cabbage seed weevil is sensed primarily by contact chemoreception at the sensilla chaetica of the antennae, and the electrophysiological responses recorded from these gustatory sensilla are of value as the basis of a bioassay to assist identification of the active constituent(s) of the pheromone.
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