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  • ANT-XXXI/3; Calculated; Chlorophyll a, size fraction 〉 2 µm; Chlorophyll a, size fraction 0.2-2 µm; Chlorophyll a, total; co-limitation; Drake Passage; Event label; Experiment; Experimental treatment; Incubation duration; Laboratory fluorometer, Turner, Trilogy; Membrane pump; MP; Phytoplankton composition; Polarstern; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; PS97; PS97/043-1; PS97/087-4; Scotia Sea; Southern Ocean; SPP1158; trace elements  (1)
  • ANT-XXXIII/3; carbon fixation; Drake Passage; Fe:C ratio; growth; ICP-MS; Inductively coupled plasma mass spectrometry; In situ pump; iron; Iron, cellular quota; ISP; manganese; Manganese, cellular quota; photophysiology; Phytoplankton; Polarstern; PS112; PS112_26-1; Southern Ocean; Trace metal; Treatment  (1)
  • Alkalinity, total; Alkalinity, total, standard deviation; Alloxanthin; Alloxanthin, standard deviation; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, particulate; Carbon, organic, particulate, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlorophyll a; Chlorophyll a, standard deviation; Chlorophyll c2; Chlorophyll c2, standard deviation; Chromista; Cobalt/Carbon ratio; Cobalt/Carbon ratio, standard deviation; Connectivity between photosystem II; Connectivity between photosystem II, standard deviation; Copper/Carbon ratio; Copper/Carbon ratio, standard deviation; Cryptophyta; Diadinoxanthin; Diadinoxanthin, standard deviation; Electron transport rate, absolute; Electron transport rate, absolute, standard deviation; Elemental analyzer, HEKAtechGmbH, Euro EA; Fluorometer, fast repetition rate; FRRF; Fucoxanthin; Fucoxanthin, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Functional absorption cross sections of photosystem II reaction centers; Functional absorption cross sections of photosystem II reaction centers, standard deviation; Functional photosystem II reaction centers; Functional photosystem II reaction centers, standard deviation; Geminigera cryophila; Growth/Morphology; Growth rate, standard deviation; Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments; Inorganic toxins; Iron, cellular quota; Iron, cellular quota, standard deviation; Iron/Carbon ratio; Iron/Carbon ratio, standard deviation; Irradiance; Laboratory experiment; Laboratory strains; Light microscopy (Utermöhl 1958); Light saturation point; Light saturation point, standard deviation; Light use efficiency; Light use efficiency, standard deviation; Manganese/Carbon ratio; Manganese/Carbon ratio, standard deviation; Maximal electron transport rate; Maximal electron transport rate, standard deviation; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, recovery; Maximum photochemical quantum yield of photosystem II, recovery, standard deviation; Maximum photochemical quantum yield of photosystem II, standard deviation; Nitrogen, organic, particulate; Non photochemical quenching; Non photochemical quenching, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate organic carbon, production, standard deviation; Particulate organic nitrogen production, standard deviation; Pelagos; pH; pH, standard deviation; Phosphate; Phytoplankton; Phytoplankton growth rate; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Production of particulate organic carbon; Pseudo-nitzschia subcurvata; Reverse phase HPLC (High Performance Liquid Chromatography); Salinity; Silicate; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperature, water; Treatment: dissolved iron; Treatment: partial pressure of carbon dioxide; Type; Zinc/Carbon ratio; Zinc/Carbon ratio, standard deviation  (1)
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  • 1
    Publication Date: 2024-05-13
    Description: Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C. Chlorophyll a samples were taken at the beginning and the end of both experiments. In order to compare the contribution of large (〉2 μm) relative to small cells (0.2-2 μm), 250 mL (on average) of samples were filtered onto 0.2 μm (for the total fraction) and 2 µm (for the large fraction) polycarbonate filters, hence the small fraction was calculated as the difference of the total and the large fraction. All samples were directly flash frozen into liquid nitrogen (N~2~) and then stored at −80 ̊C in the dark until further analysis. After being homogenized, samples were extracted in 90% acetone for 24h at 4 ̊C in the dark and analyzed fluorometrically on a Trilogy Fluorometer.
    Keywords: ANT-XXXI/3; Calculated; Chlorophyll a, size fraction 〉 2 µm; Chlorophyll a, size fraction 0.2-2 µm; Chlorophyll a, total; co-limitation; Drake Passage; Event label; Experiment; Experimental treatment; Incubation duration; Laboratory fluorometer, Turner, Trilogy; Membrane pump; MP; Phytoplankton composition; Polarstern; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; PS97; PS97/043-1; PS97/087-4; Scotia Sea; Southern Ocean; SPP1158; trace elements
    Type: Dataset
    Format: text/tab-separated-values, 164 data points
    Location Call Number Expected Availability
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  • 2
    Publication Date: 2024-05-13
    Description: For determination of the cellular trace metal content, at the end of the experiment 400 mL of water were filtered onto 0.2 μm TMC-cleaned polycarbonate filters (EMD Millipore, Darmstadt, Germany) and then rinsed for 15 min with a 0.1 M oxalic acid wash to remove cell surface bound trace metals. Finally, the filters were rinsed with filtered seawater and stored in 5 mL TMC polyfluoralkoxy vials until further analysis. Samples were analyzed with an inductively coupled plasma mass spectrometry (ICP-MS) following a digestion with nitric acid (HNO3) and HF.
    Keywords: ANT-XXXIII/3; carbon fixation; Drake Passage; Fe:C ratio; growth; ICP-MS; Inductively coupled plasma mass spectrometry; In situ pump; iron; Iron, cellular quota; ISP; manganese; Manganese, cellular quota; photophysiology; Phytoplankton; Polarstern; PS112; PS112_26-1; Southern Ocean; Trace metal; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 48 data points
    Location Call Number Expected Availability
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  • 3
    Publication Date: 2024-05-22
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Alloxanthin; Alloxanthin, standard deviation; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, particulate; Carbon, organic, particulate, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlorophyll a; Chlorophyll a, standard deviation; Chlorophyll c2; Chlorophyll c2, standard deviation; Chromista; Cobalt/Carbon ratio; Cobalt/Carbon ratio, standard deviation; Connectivity between photosystem II; Connectivity between photosystem II, standard deviation; Copper/Carbon ratio; Copper/Carbon ratio, standard deviation; Cryptophyta; Diadinoxanthin; Diadinoxanthin, standard deviation; Electron transport rate, absolute; Electron transport rate, absolute, standard deviation; Elemental analyzer, HEKAtechGmbH, Euro EA; Fluorometer, fast repetition rate; FRRF; Fucoxanthin; Fucoxanthin, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Functional absorption cross sections of photosystem II reaction centers; Functional absorption cross sections of photosystem II reaction centers, standard deviation; Functional photosystem II reaction centers; Functional photosystem II reaction centers, standard deviation; Geminigera cryophila; Growth/Morphology; Growth rate, standard deviation; Inductively coupled plasma mass spectrometer (ICP-MS), Attom, Nu Instruments; Inorganic toxins; Iron, cellular quota; Iron, cellular quota, standard deviation; Iron/Carbon ratio; Iron/Carbon ratio, standard deviation; Irradiance; Laboratory experiment; Laboratory strains; Light microscopy (Utermöhl 1958); Light saturation point; Light saturation point, standard deviation; Light use efficiency; Light use efficiency, standard deviation; Manganese/Carbon ratio; Manganese/Carbon ratio, standard deviation; Maximal electron transport rate; Maximal electron transport rate, standard deviation; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, recovery; Maximum photochemical quantum yield of photosystem II, recovery, standard deviation; Maximum photochemical quantum yield of photosystem II, standard deviation; Nitrogen, organic, particulate; Non photochemical quenching; Non photochemical quenching, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate organic carbon, production, standard deviation; Particulate organic nitrogen production, standard deviation; Pelagos; pH; pH, standard deviation; Phosphate; Phytoplankton; Phytoplankton growth rate; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Production of particulate organic carbon; Pseudo-nitzschia subcurvata; Reverse phase HPLC (High Performance Liquid Chromatography); Salinity; Silicate; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperature, water; Treatment: dissolved iron; Treatment: partial pressure of carbon dioxide; Type; Zinc/Carbon ratio; Zinc/Carbon ratio, standard deviation
    Type: Dataset
    Format: text/tab-separated-values, 8948 data points
    Location Call Number Expected Availability
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