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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning and analysis of nre, the major nitrogen regulatory gene of Penicillium chrysogenum (1995)
    Springer
    Current genetics 27 (1995), S. 150-158 
    Details
    ISSN: 1432-0983
    Keywords: Penicillium ; Nitrogen regulation ; Polymerase chain reaction ; Cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated the Penicillium chrysogenum nre gene which is homologous to the major nitrogen regulatory genes areA from Aspergillus nidulans and nit-2 from Neurospora crassa. Overall, nre shows 60% identity to areA and 30% identity to nit-2 at the amino-acid level. The gene encodes a protein of 835 amino-acid residues and contains a single Cys2/Cys2-type zinc finger with an adjacent basic region and a putative acidic activation region. In the DNA-binding domain, 98% of the amino-acid residues are identical in nre, areA and nit-2. The nre gene has been shown to be functional in N. crassa by heterologous complementation of a nit-2 mutant. Growth tests indicated that transformants could utilize nitrate, amino-acids, purines and amides as sole nitrogen sources. Nitrate reductase activity assays performed with transformants demonstrated that nitrogen control was completely normal. Complementation of N. crassa nit-2 mutants with 5′-deletion clones of nre suggests the possible presence of an internal promoter within the coding region. Northern analysis and ribonuclease protection assays of total cellular RNA indicated that nre encodes a 3.2-kb transcript which is reduced in content under conditions of nitrogen repression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00313429
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Equilibrium structure of hydrogen-bonded polymer blends (1998)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 36 (1998), S. 2745-2750 
    Details
    ISSN: 0887-6266
    Keywords: polymer blends ; hydrogen bonding ; small-angle neutron scattering ; Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Deuterium-labeled polystyrene modified by random distributions of the comonomer p-(1,1,1,3,3,3-hexaflouro-2-hydroxyisopropyl)-α-methyl-styrene [DPS(OH)] has been blended with poly(butyl methacrylate) (PBMA) and studied with small-angle neutron scattering (SANS). Miscibility is induced via hydrogen bonding between the DPS(OH) hydroxyl group and PBMA carbonyl groups. The data suggest that the nature of the miscible-phase structure in these blends differs from that of the usual homopolymer blends at small scattering angles, which we attribute to the short-range site specific nature of the hydrogen bond interaction. © 1998 John Wiley & Sons, Inc. J Polym Sci B: Polym Phys 36: 2745-2750, 1998
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 3
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    Air transportation in the California Corridor of 2010 (1989)
    In:  CASI
    Details
    Publication Date: 2017-03-17
    Description: The topic of the 1988-1989 NASA/USRA Advanced Design Project at California Polytechnic State University, San Luis Obispo, was the development of an air transportation system to meet the needs of the California Corridor for the year 2010. As aircraft design is taught by two instructors having different philosophies about the teaching process, the two classes took different approaches to address the problem. The first part of this summary (California Air Transit System) represents the work done by the students of Professor A. E. Andreoli, who followed a systems approach, emphasizing the determination of the proper mission. The second part of the summary (Four Aircraft to Service the California Corridor) contains the four aircraft designed by Dr. D. R. Sandlin's class based on specifications determined from work done in previous years.
    Keywords: AIR TRANSPORTATION AND SAFETY
    Type: USRA, NASA(USRA University Advanced Design Program Fifth Annual Summer Conference; p 267-271
    Format: text
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    NASA TECHNICAL REPORTS
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