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  • AGE; DEPTH, sediment/rock; Event label; GC; Globigerina bulloides, Magnesium/Calcium ratio; Gravity corer; Inductively coupled plasma - mass spectrometry (ICP-MS); MV99_GC41; MV99_PC14; North Pacific; Sea surface temperature, annual mean; SST from Mg/Ca ratios  (1)
  • Computational Methods, Nucleic Acid Enzymology  (1)
  • Kerala  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Agroforestry systems 24 (1993), S. 203-213 
    ISSN: 1572-9680
    Keywords: Kerala ; homegardens ; agroforestry ; low input agriculture ; sustainability ; architectural analysis ; human ecosystem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An analogue approach to analysing the traditional homegardens of Keralavis-à-vis natural climax ecosystems is adopted. The traditional homegarden is apparently a climax ecosystem, where ecological succession is consciously manipulated by human beings. High intensity of vertical and horizontal space use, the highly dynamic chronological structure and the capacity to perform essential ecological processes make this ecosystem relatively sustainable. Homegardens in Kerala effectively serve as human ecosystems with their low input demand, staggered supply of outputs, and enhancement of habitat quality. However, recent trends in agrarian structure and the high market orientation exert pressures on the homegarden, and its sustainability as a human ecosystem is in question.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2023-02-12
    Keywords: AGE; DEPTH, sediment/rock; Event label; GC; Globigerina bulloides, Magnesium/Calcium ratio; Gravity corer; Inductively coupled plasma - mass spectrometry (ICP-MS); MV99_GC41; MV99_PC14; North Pacific; Sea surface temperature, annual mean; SST from Mg/Ca ratios
    Type: Dataset
    Format: text/tab-separated-values, 866 data points
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  • 3
    Publication Date: 2016-12-17
    Description: Gene 32 protein (gp32) is the single-stranded (ss) DNA binding protein of the bacteriophage T4. It binds transiently and cooperatively to ssDNA sequences exposed during the DNA replication process and regulates the interactions of the other sub-assemblies of the replication complex during the replication cycle. We here use single-molecule FRET techniques to build on previous thermodynamic studies of gp32 binding to initiate studies of the dynamics of the isolated and cooperative binding of gp32 molecules within the replication complex. DNA primer/template (p/t) constructs are used as models to determine the effects of ssDNA lattice length, gp32 concentration, salt concentration, binding cooperativity and binding polarity at p/t junctions. Hidden Markov models (HMMs) and transition density plots (TDPs) are used to characterize the dynamics of the multi-step assembly pathway of gp32 at p/t junctions of differing polarity, and show that isolated gp32 molecules bind to their ssDNA targets weakly and dissociate quickly, while cooperatively bound dimeric or trimeric clusters of gp32 bind much more tightly, can ‘slide’ on ssDNA sequences, and exhibit binding dynamics that depend on p/t junction polarities. The potential relationships of these binding dynamics to interactions with other components of the T4 DNA replication complex are discussed.
    Keywords: Computational Methods, Nucleic Acid Enzymology
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
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