ISSN:
1573-0603
Keywords:
Determination
;
Differentiation
;
Immortalization
;
Lineage
;
Stem cells
;
Teratocarcinoma
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A method underlying a strategy for immortalization of cell lines from multipotential teratocarcinoma cells is described. Embryonal carcinoma (EC) cells are transformed by a plasmid pK4 in which SV40 oncogenes are under the control of the adenovirus ElA promoter; pK4-transformed EC cell lines are selected and induced to differentiate. The expression of SV40 at the onset of differentiation allows the immortalization of permanent cell lines corresponding to early embryonic derivatives of each lineage which can be cloned and selected.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00986658
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