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  • Life Sciences (General)  (10)
  • AERODYNAMICS  (4)
  • Astrophysics  (4)
  • AIRCRAFT PROPULSION AND POWER  (3)
  • 1
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    Elliptical-P cells in the avian perilymphatic interface of the Tegmentum vasculosum (1995)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: Elliptical cells (E-P) are present at the perilymphatic interface lumen (PIL) of the lagena. The E-P cells often separate from the tegmentum vasculosum (TV) and have touching processes that form a monolayer between the K+ rich perilymph and the Na+ rich endolymph, similar to the mammalian Reissner's membrane. We examined the TV of chicks (Gallus domesticus) and quantitated the expression of anti-S100 alphaalphabetabeta and S100 beta. There was a 30% increase of S100 beta saturation in the light cells facing the PIL when compared to other TV light cells. We show that: (1) the dimer anti- S100 alphaalphabetabeta and the monomer anti-S100 beta are expressed preferentially in the light cells and the E-P cells of TV; (2) expression of S100 beta is higher in light cells facing the PIL than in adjacent cells; (3) the expression of the dimer S100 alphaalphabetabeta and monomer S100 beta overlaps in most inner ear cell types, including the cells of the TV, most S100 alphaalphabetabeta positive cells express S 100 beta, but S100 beta positive cells do not always express S100 alphaalphabetabeta; and (4) the S100 beta expression in light cells, the abundant Na+-K+ ATPase on dark cells of the TV, and previously demonstrated co-localization of S100 beta/GABA in sensory cells suggest that S100 beta could have, in the inner ear, a dual neurotrophic-ionic modulating function.
    Keywords: Life Sciences (General)
    Type: Scanning microscopy (ISSN 0891-7035); Volume 9; 4; 1207-21; discussion 1221-2
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  • 2
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    Post-embedding tem signal-to-noise ratio of S-100 (1994)
    In:  Other Sources
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    Publication Date: 2011-08-24
    Description: We assessed the reactivity of purified S-100 antiserum in immuno-electron microscopy by counting the number of gold particles per microns 2 over inner ear tissues embedded in different media. Sections containing predominantly Schwann's cell cytoplasm and nucleus, afferent fiber axoplasm and myelin sheath of chick cochleae were reacted with anti-S-100 IgG, an antibody to a calcium binding protein of neuronal tissues, then labeled with anti-IgG-gold conjugate. This investigation was conducted because previously published procedures, unmodified, did not yield acceptable results. Preparation of all specimens was identical. Only the medium (PolyBed 812, Araldite or Spurr epoxies; and LR White, LR Gold or Lowicryl plastics) was changed. The medium was made the changing variable because antigens available in post-embedding immuno-electron microscopy are decreased by heat, either used and/or released during polymerization of the embedding medium. The results indicate that: (a) none of the embedding media above provided optimal signal-to-noise ratio for all parts of the nerve stained in the same section; (b) aggregation of gold particles over cells was highest in embedding media with high background labeling over areas devoid of tissue (noise); (c) aggregation occurred randomly throughout both cellular and acellular regions; and (d) particles aggregated less and were distributed more evenly in tissues from media yielding good ultrastructural integrity.
    Keywords: Life Sciences (General)
    Type: Hearing research (ISSN 0378-5955); Volume 73; 2; 195-202
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  • 3
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    Expression of S100 beta in sensory and secretory cells of the vertebrate inner ear (1995)
    In:  Other Sources
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    Publication Date: 2011-08-24
    Description: We evaluated anti-S100 beta expression in the chick (Gallus domesticus) inner ear and determined that: 1) the monomer anti-S100 beta is expressed differentially in the vestibular and auditory perikarya; 2) expression of S100 beta in the afferent nerve terminals is time-related to synapse and myelin formation; 3) the expression of the dimer anti-S100 alpha alpha beta beta and monomer anti-S100 beta overlaps in most inner ear cell types. Most S100 alpha alpha beta beta positive cells express S100 beta, but S100 beta positive cells do not always express S100 alpha alpha beta beta. 4) the expression of S100 beta is diffused over the perikaryal cytoplasm and nuclei of the acoustic ganglia but is concentrated over the nuclei of the vestibular perikarya. 6) S100 beta is expressed in secretory cells, and it is co-localized with GABA in sensory cells. 7) Color thresholding objective quantitation indicates that the amount of S100 beta was higher (mean 22, SD +/- 4) at E19 than at E9 (mean 34, SD +/- 3) in afferent axons. 8) Moreover, S100 beta was unchanged between E11-E19 in the perikaryal cytoplasm, but did change over the nuclei. At E9, 74%, and at E21, 5% of vestibular perikarya were positive. The data suggest that S100 beta may be physically associated with neuronal and ionic controlling cells of the vertebrate inner ear, where it could provide a dual ionic and neurotrophic modulatory function.
    Keywords: Life Sciences (General)
    Type: Cellular and molecular biology (Noisy-le-Grand, France) (ISSN 0145-5680); Volume 41; 2; 213-25
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  • 4
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    Microgravity in the STS-29 space shuttle discovery affected the vestibular system of chick embryos (1996)
    In:  Other Sources
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    Publication Date: 2011-08-24
    Description: Out of 32 embryos flown (16 @ E2 + 16 @ E9) for 5 days, 16 survived. All sixteen E2 were dead at landing. Eight were opened and eight were incubated at 1.0G. Autopsy showed that 4 E2 survived over 24 hours in space. Eight E14 hatched without anatomical malformations, and 8 E14 were fixed. The height of the macular epithelia was 31 mu m (mean) in control and 26 mu m in flight chicks. The cross-sectional area of macular nuclei of control was 17 mu m(2) for hair cells and 14 mu m(2) in supporting cells. In flight, cross-sectional area was 17 mu m(2) in hair cells and 15 mu m(2) in supporting cells (n=250). The shape factor of cartilage cells (1.0 = perfect circle) between control (mean = 0.70) and flight (mean = 0.72), and the area of cartilaginous cells between controls (mean = 9 mu m(2)) and flight (mean = 9 mu m(2)) did not differ (n=300). The nuclei of support cells were closer to the basement membrane in flight than in control chicks. The immunoreactivity of otoconia with anti keratan, fibronectin or chrondroitin sulfate was not different between flight and control ears. There were more afferent fibers inside the macular epithelia of flight (p〈0.05) than control. Three of 8 flight animals had elevated vestibular thresholds (VT), with normal mean response amplitudes and latencies. Modified afferent innervation patterns requiring weeks to compensate are sufficient to elevate VT, and should be investigated further. Other reversible (sublethal) microgravity effects on sensory epithelia (vacuoles, swelling, etc) require quantification.
    Keywords: Life Sciences (General)
    Type: Histology and histopathology (ISSN 0213-3911); Volume 11; 2; 407-26
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  • 5
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    Sensor biases effect on the estimation algorithm for performance-seeking controllers (1994)
    In:  Other Sources
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    Publication Date: 2011-08-24
    Description: The performance-seeking-control algorithm (PSC) is designed to continuously optimize the performance of propulsion systems. The PSC uses a nominal model of the propulsion system and estimates, in flight, the engine deviation parameters (EDPs) characterizing engine deviations with respect to nominal conditions. In practice, the measurement biases (or model uncertainties) may prevent the estimated EDPs from reflecting the engine's actual off-nominal condition. This factor has a direct impact on the PSC scheme exacerbated by the open-loop character of the algorithm. An observability analysis shows that the biases cannot be estimated together with the EDPs. Moreover, biases and EDPs turn out to have equivalent effects on the measurements, leaving it undecided whether the estimated EDPs represent the actual engine deviation or whether they simply reflect the measurement biases. In this article, the effects produced by unknown measurement biases over the estimation algorithm are evaluated. This evaluation allows for identification of the most critical measurements for application of the PSC algorithm to an F100 engine.
    Keywords: AIRCRAFT PROPULSION AND POWER
    Type: Journal of Propulsion and Power (ISSN 0748-4658); 10; 4; p. 527-532
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  • 6
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    Experimental and Analytical Investigation of the Transonic and Supersonic Divergence Characteristics of a Delta-Plan-Form All- Movable Control (1958)
    In:  CASI
    Details
    Publication Date: 2019-05-23
    Description: The static aeroelastic divergence characteristics of a delta-planform model of the canard control surface of a proposed air-to-ground missile have been studied both analytically and experimentally in the Mach number range from 0.6 to 3.0. The experiments indicated that divergence occurred at a nearly constant value of dynamic pressure at Mach numbers up to 1.2. At higher Mach numbers somewhat higher values of dynamic pressure were required to produce divergence. The analysis and the experiment indicate that the camber stiffness of the control surface and the stiffness of the control actuator are both important in divergence of surfaces of this type.
    Keywords: AERODYNAMICS
    Type: NACA-RM-L58E07
    Format: application/pdf
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  • 7
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    Investigation of the divergence characteristics of delta-planform canard controls (1966)
    In:  CASI
    Details
    Publication Date: 2019-05-29
    Description: Static aeroelastic divergence characteristics of delta-planform model of canard control surfaces
    Keywords: AERODYNAMICS
    Type: NASA-TR-R-235
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  • 8
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    VSAERO analysis of tip planforms for the free-tip rotor (1988)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: The results of a numerical analysis of two interacting lifting surfaces separated in the spanwise direction by a narrow gap are presented. The configuration consists of a semispan wing with the last 32 percent of the span structurally separated from the inboard section. The angle of attack of the outboard section is set independently from that of the inboard section. In the present study, the three-dimensional panel code VSAERO is used to perform the analysis. Computed values of tip surface lift and pitching moment coefficients are correlated with experimental data to determine the proper approach to model the gap region between the surfaces. Pitching moment data for various tip planforms are also presented to show how the variation of tip pitching moment with angle of attack may be increased easily in incompressible flow. Calculated three-dimensional characteristics in compressible flow at Mach numbers of 0.5 and 0.7 are presented for new tip planform designs. An analysis of sectional aerodynamic center shift as a function of Mach number is also included for a representative tip planform. It is also shown that the induced drag of the tip surface is reduced for negative incidence angles relative to the inboard section. The results indicate that this local drag reduction overcomes the associated increase in wing induced drag at high wing lift coefficients.
    Keywords: AERODYNAMICS
    Type: NASA-CR-177487 , NAS 1.26:177487
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  • 9
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    Experimental study of an independently deflected wingtip mounted on a semispan wing (1991)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: The results of a subsonic wind tunnel test of a semispan wing with an independently deflected tip surface are presented and analyzed. The tip surface was deflected about the quarter chord of the rectangular wing and accounted for 17 percent of the wing semispan. The test was conducted to measure the loads on the tip surface and to investigate the nature of aerodynamic interference effects between the wing and the deflected tip. Results are presented for two swept tip surfaces of similar planform but different airfoil distributions. The report contains plots of tip lift, drag, and pitching moment for various Reynolds numbers and tip deflection angles with respect to the inboard wing. Oil flow visualization photographs for a typical Reynolds number are also included. Important aerodynamic parameters such as lift and pitching moment slopes and tip aerodynamic center location are tabulated. A discussion is presented on the relationship between tip experimental data acquired in a steady flow and the prediction of unsteady tip motion at fixed wing angles of attack.
    Keywords: AERODYNAMICS
    Type: NASA-TM-102842 , A-90210 , NAS 1.15:102842
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  • 10
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    On the estimation algorithm used in adaptive performance optimization of turbofan engines (1993)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: The performance seeking control algorithm is designed to continuously optimize the performance of propulsion systems. The performance seeking control algorithm uses a nominal model of the propulsion system and estimates, in flight, the engine deviation parameters characterizing the engine deviations with respect to nominal conditions. In practice, because of measurement biases and/or model uncertainties, the estimated engine deviation parameters may not reflect the engine's actual off-nominal condition. This factor has a necessary impact on the overall performance seeking control scheme exacerbated by the open-loop character of the algorithm. The effects produced by unknown measurement biases over the estimation algorithm are evaluated. This evaluation allows for identification of the most critical measurements for application of the performance seeking control algorithm to an F100 engine. An equivalence relation between the biases and engine deviation parameters stems from an observability study; therefore, it is undecided whether the estimated engine deviation parameters represent the actual engine deviation or whether they simply reflect the measurement biases. A new algorithm, based on the engine's (steady-state) optimization model, is proposed and tested with flight data. When compared with previous Kalman filter schemes, based on local engine dynamic models, the new algorithm is easier to design and tune and it reduces the computational burden of the onboard computer.
    Keywords: AIRCRAFT PROPULSION AND POWER
    Type: NASA-TM-4551 , H-1908 , NAS 1.15:4551 , AIAA PAPER 93-1823 , AIAA Joint Propulsion Conference; Jun 28, 1993 - Jul 01, 1993; Monterey, CA; United States
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