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Depth profiling a III–V multilayered structure with an excimer laser (1994)

Bourne, O. L. ; Hart, D'Arcy ; Rayner, D. M. ; [et al.]

Springer

Applied physics 59 (1994), S. 295-297

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ISSN: 1432-0630

Keywords: 81.60 ; 82.65 ; 82.80

Source: Springer Online Journal Archives 1860-2000

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics

Notes: Abstract A multilayered structure of GaAs and AlGaAs was depth profiled using the technique of digital etching. A single excimer laser (KrF) was used to control the etch rate and to identify each layer by monitoring the Ga ions generated during the desorption process. The Ga ions were the only ions observed and were only generated when the photon flux was in the GaAs layer. The etch rate, 0.9 monolayers (2.5 Å) per pulse, was constant with depth. The overall layer recognition resolution was 45 monolayers.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00348233

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Rapid chemiluminescent detection of mRNAs on Northern blots with digoxigenin end-labelled oligonucleotides (1995)

Trayhurn, Paul ; Duncan, Jacqueline S. ; Nestor, Antoinette ; [et al.]

Weinheim : Wiley-Blackwell

Electrophoresis 16 (1995), S. 341-344

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ISSN: 0173-0835

Keywords: Chemiluminescence ; Digoxigenin ; Northern blot ; Oligonucleotides ; mRNA ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: A simplified, nonradioactive procedure for the detection of specific mRNAs on Northern blots has been developed, utilizing digoxigenin-labelled oligonucleotides and chemiluminescence. Antisense oligonucleotide (30-35 mer) probes were designed and synthesised based on published cDNA and gene sequences. These probes were end-labelled (5′) with digoxigenin. Total RNA was fractionated by agarose gel electrophoresis and capillary blotted onto positively charged nylon membranes. After hybridization, the mRNA/digoxigenin-labelled oligonucleotide complex was detected by a chemiluminescence-based method using disodium 3-(4-methoxyspiro-[1,2-dioxetane-3-2′(5′chloro)-tricyclo[3.3.1.13.7]decane]-4-yl)phenyl phosphate (CSPD) as substrate. The advantages of this simplified technique for detecting mRNAs in physiological and nutritional studies are described.

Additional Material: 3 Ill.