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  • mitochondrial DNA  (2)
  • 74.60e  (1)
  • Brassica campestris  (1)
  • Mitotic index
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Keywords
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  • 1
    Electronic Resource
    Electronic Resource
    Growth characteristics and transformability of soybean embryogenic cultures (1998)
    Springer
    Plant cell reports 17 (1998), S. 765-772 
    Details
    ISSN: 1432-203X
    Keywords: Key words Microprojectile bombardment ; Mitotic index ; Somatic embryogenesis ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Embryogenic cultures of soybean [Glycine max (L.) Merr. cv. Jack and Asgrow A2872] were established in liquid Finer and Nagasawa medium, maintained by transfer to fresh medium at biweekly intervals, and subjected to microprojectile bombardment over time. Cultures were not amenable to transformation until they were at least 6 months old. Over time, different cell lines of the same genotype acquired very different culture phenotypes. Histological analysis of cell lines differing in transformation ability showed that the most transformable cultures had cytoplasmic-rich cells in the outermost layers of the tissue. In contrast, the outer layers of less transformable cultures contained cells with prominent vacuoles. Although fresh weight accumulation of the cultures was curvilinear during the 2-week subculture period, a burst of mitotic activity was evident shortly after transfer to fresh medium. This activity usually lasted from the 2nd to the 6th day following subculture, and peaked on the 4th day. Tissues at or near this stage always produced more transient expression of a reporter gene than did bombardments at other times. In addition, the cell lines most amenable to transformation also exhibited the highest mitotic index. Thus any treatment to increase the mitotic index, especially when the cell lines are less than 6 months old, may facilitate the transformation of cell lines from which efficient recovery of transgenic plants is still possible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050480
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  • 2
    Electronic Resource
    Electronic Resource
    Andreev reflection on the Ag−BaPb1−x Bi x O3 microconstriction: Temperature and magnetic field dependence (1997)
    Springer
    Journal of low temperature physics 106 (1997), S. 291-296 
    Details
    ISSN: 1573-7357
    Keywords: 74.50 ; 74.60e
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The point-contact spectroscopy has been performed on the normal metal-superconductor microconstriction Ag−BaPb1−xBixO3. The conductance curves of our junctions reveal almost ideal Andreev reflection behavior described by theBlonder-Tinkham-Klapwijk theory, with the in-gap conductance twice higher than the conductance at higher voltages. We studied the effect of the temperature and magnetic field on the Andreev reflection. The temperature dependence of the energy gap (determined from the BTK theory) follows the BCS prediction. The value of the reduced gap yields a weak coupling strength in BaPb1−xBixO3. ¿From magnetic measurements up to 10 Tesla the temperature dependence of the upper critical field Hc2 has been determined and found to follow the Werthamer-Helfand-Hohenberg prediction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02399628
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic evidence for the existence of cryptic species in theAnopheles albitarsis complex in Brazil: Allozymes and mitochondrial DNA restriction fragment length polymorphisms (1993)
    Springer
    Biochemical genetics 31 (1993), S. 97-112 
    Details
    ISSN: 1573-4927
    Keywords: Anopheles albitarsis ; allozymes ; mitochondrial DNA ; restriction fragment length polymorphism ; cryptic species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Allozyme and mitochondrial DNA (mtDNA) restriction studies were undertaken to determine the extent of genetic divergence among field populations ofAnopheles albitarsis in Brazil. Two sympatric species,An. deaneorum andAn. marajoara, were identified in collections from Costa Marques (CM), Rondonia. Genetic evidence includes (1) the presence of two types of individuals, each with diagnostic allelic clusters (forHad-1, Pgi-1, Pep-1, Mpi-1, andIdh-1), (2) a deficiency of heterozygotes, and (3) characteristic mtDNA haplotypes. In addition, two allopatric cryptic species ofAn. marajoara were identified, one from Iguape (An. marajoara form IG), Sao Paulo state, and the other from the Island of Marajo (An. marajoara form MA). Though form IG and form-MA resemble form CM in wing spot morphology, they differ from it in diagnostic allozymes and mtDNA haplotypes.An. marajoara form CM had a higher variability (mean heterozygosity,H=0.22, and percentage of polymorphic loci,P=66.7) than did form IG and form MA (H=0.08 in both, andP=25.0 and 33.3, respectively). Form MA and form IG are genetically more similar to each other than both are to form CM. Based on wing morphology, estimates ofF statistics, and genetic similarities, we propose thatAn. albitarsis in Brazil is a species complex. It comprises at least two morphologically distinguishable species: (1)An. deaneorum (currently one taxon) and (2) theAn. marajoara species complex, which further consists of at least three cryptic forms,marajoara form MA,marajoara form IG, andmarajoara form CM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00553604
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic evidence for the existence of cryptic species in theAnopheles albitarsis complex in Brazil: Allozymes and mitochondrial DNA restriction fragment length polymorphisms (1993)
    Springer
    Biochemical genetics 31 (1993), S. 97-112 
    Details
    ISSN: 1573-4927
    Keywords: Anopheles albitarsis ; allozymes ; mitochondrial DNA ; restriction fragment length polymorphism ; cryptic species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Allozyme and mitochondrial DNA (mtDNA) restriction studies were undertaken to determine the extent of genetic divergence among field populations ofAnopheles albitarsis in Brazil. Two sympatric species,An. deaneorum andAn. marajoara, were identified in collections from Costa Marques (CM), Rondonia. Genetic evidence includes (1) the presence of two types of individuals, each with diagnostic allelic clusters (forHad-1, Pgi-1, Pep-1, Mpi-1, andIdh-1), (2) a deficiency of heterozygotes, and (3) characteristic mtDNA haplotypes. In addition, two allopatric cryptic species ofAn. marajoara were identified, one from Iguape (An. marajoara form IG), Sao Paulo state, and the other from the Island of Marajo (An. marajoara form MA). Though form IG and form-MA resemble form CM in wing spot morphology, they differ from it in diagnostic allozymes and mtDNA haplotypes.An. marajoara form CM had a higher variability (mean heterozygosity,H=0.22, and percentage of polymorphic loci,P=66.7) than did form IG and form MA (H=0.08 in both, andP=25.0 and 33.3, respectively). Form MA and form IG are genetically more similar to each other than both are to form CM. Based on wing morphology, estimates ofF statistics, and genetic similarities, we propose thatAn. albitarsis in Brazil is a species complex. It comprises at least two morphologically distinguishable species: (1)An. deaneorum (currently one taxon) and (2) theAn. marajoara species complex, which further consists of at least three cryptic forms,marajoara form MA,marajoara form IG, andmarajoara form CM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02399823
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  • 5
    Electronic Resource
    Electronic Resource
    In vitro vegetative propagation of Chinese cabbage (1991)
    Springer
    Plant cell, tissue and organ culture 26 (1991), S. 135-139 
    Details
    ISSN: 1573-5044
    Keywords: Brassica campestris ; cotyledon ; propagation rate ; seed set
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Explants from cotyledons, axillary buds, inflorescence stems and flower buds of Brassica campestris ssp. pekinensis (Lour.) Olsson (Chinese cabbage, cv. Wongbok) were cultured on MS medium with growth regulators. Multiple shoots were obtained from cotyledons, axillary buds and flower buds but not from inflorescence stems. Propagation of shoots from cotyledons was more successful than from axillary buds and flower buds. The vegetative propagation rates varied amongst clones derived from cotyledons of the same cultivar and seed lot. The propagation rates of the cotyledon-derived material followed a normal distribution with an average propagation rate of 2.6 shoots per two weeks subculture when cultured on MS media plus 44.4 μm benzyladenine (BA) and 14.8 μm β-indolebutyric acid (IBA). Shoots from three clones were cultured on MS medium with nine different concentrations of BA. The concentration of BA which promoted the highest rate of shoot propagation varied for the three clones and was in the range 44.4 to 177.6 μm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036117
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