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  • 550 - Earth sciences  (1)
  • Retrotransposon  (1)
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  • 1
    ISSN: 1617-4623
    Keywords: Key wordsDictyostelium ; tRNA gene ; Retrotransposon ; Genome evolution ; Dictyostelium Genome Project
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Retrotransposable elements are genetic entities which move and replicate within host cell genomes. We have previously reported on the structures and genomic distributions of two non-long terminal repeat (non-LTR) retrotransposons, DRE and Tdd-3, in the eukaryotic microorganism Dictyostelium discoideum. DRE elements are found inserted upstream, and Tdd-3 elements downstream, of transfer RNA (tRNA) genes with remarkable position and orientation specificities. The data set currently available from the Dictyostelium Genome Project led to the characterisation of two repetitive DNA elements which are related to the D. discoideum non-LTR retrotransposon Tdd-3 in both their structural properties and genomic distributions. It appears from our data that in the D. discoideum genome tRNA genes are major targets for the insertion of mobilised non-LTR retrotransposons. This may be interpreted as the consequence of a process of coevolution, allowing a viable population of retroelements to transpose without being deleterious to the small microbial host genome which carries only short intergenic DNA sequences. A new nomenclature is introduced to designate all tRNA gene-targeted non-LTR retrotransposons (TREs) in the D. discoideum genome. TREs inserted 5′ and 3′ of tRNA genes are named TRE5 and TRE3, respectively. According to this nomenclature DRE and Tdd-3 are renamed TRE5-A and TRE3-A, respectively. The new retroelements described in this study are named TRE3-B (formerly RED) and TRE3-C.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2020-02-12
    Description: The concentrations of H2, O2, CO2 and concentrations and isotopic composition of the noble gases (including 222Rn), N2, CH4, and higher hydrocarbons dissolved in 4000 m deep-seated fluids from a 12-month fluid production test in the KTB pilot hole were analyzed. This determination of the gas geochemistry during the test in combination with the knowledge of the hydraulic data provides relevant information about the fluid hydraulics of the deep system. All gas concentrations and isotopic signatures, except for 222Rn showed constancy during the course of the test. This, in combination with large fluid flow rates at a moderate water table drawdown, imply an almost infinite fluid reservoir in 4000 m depth. From the change in 222Rn activity as a function of pump rate, the contribution of smaller and wider pores to the overall fluid flow in an aquifer can be deduced. This 222Rn-activity monitoring proved therefore to be a valuable instrument for the qualitative observation of the scavenging of pore and fracture surfaces, a hydraulic feature invisible to standard hydraulic testing tools. The observance of this scavenging effect is due to (i) the continuous on-line geochemical monitoring, (ii) the durability of the test, (iii) a change in pump rate during the course of the test, and (iv) due to the short half-life of 222Rn. The fluids have a 5.9% mantle. He component, and a delta21Ne excess of 14%, and a noble gas model age of about (5.5— 6.2) ± 2.0 Myr. The mean N2/Ar-ratio of 516 and delta15N-data of about +1.5% indicates sedimentary or metamorphic origin of N2. The hydrocarbons, amounting to 33 vol. % in the gas phase, are derived from thermal decomposition of marine organic matter of low maturity. But a key question, the identification of the potential source region of the fluids and the migration pathway, is still unidentified.
    Keywords: 550 - Earth sciences
    Type: info:eu-repo/semantics/article
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