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  • 1
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    American Association for the Advancement of Science (AAAS)
    Publikationsdatum: 1985-11-29
    Beschreibung: Clathrin-coated membranes are intimately associated with a variety of protein transport processes in eukaryotic cells, yet no direct test of clathrin function has been possible. The data presented demonstrate that Saccharomyces cerevisiae does not require clathrin for either cell growth or protein secretion. Antiserum to the yeast clathrin heavy chain has been used to isolate a molecular clone of the heavy chain gene (CHC1) from a library of yeast DNA in lambda gt11. Clathrin-deficient mutant yeast have been obtained by replacing the single chromosomal CHC1 gene with a disrupted version of the cloned DNA. Cells harboring a nonfunctional chc1 allele produce no immunoreactive heavy chain polypeptide, and vesicles prepared from mutant cells are devoid of clathrin heavy and light chains. Although clathrin-deficient cells grow two to three times more slowly than normal, secretion of invertase occurs at a nearly normal rate. Therefore protein transport through the secretory pathway is not obligately coupled to the formation of clathrin-coated vesicles.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Payne, G S -- Schekman, R -- New York, N.Y. -- Science. 1985 Nov 29;230(4729):1009-14.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2865811" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Biological Transport ; *Cell Physiological Phenomena ; Clathrin/genetics/immunology/*physiology ; Coated Pits, Cell-Membrane/*physiology ; Endosomes/*physiology ; Eukaryotic Cells/*physiology ; Genes ; Genes, Fungal ; Genetic Engineering ; Glycoside Hydrolases/secretion ; Macromolecular Substances ; Molecular Weight ; Proteins/*secretion ; Saccharomyces cerevisiae/genetics ; beta-Fructofuranosidase
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Geometriae dedicata 52 (1994), S. 227-253 
    ISSN: 1572-9168
    Schlagwort(e): 51E15 ; 51E25
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract This paper is a survey on the existence and non-existence of ovoids and spreads in the known finite generalized quadrangles. It also contains the following new results. We prove that translation generalized quadrangles of order (s,s 2), satisfying certain properties, have a spread. This applies to three known infinite classes of translation generalized quadrangles. Further a new class of ovoids in the classical generalized quadranglesQ(4, 3 e ),e≥3, is constructed. Then, by the duality betweenQ(4, 3 e ) and the classical generalized quadrangleW (3 e ), we get line spreads of PG(3, 3 e ) and hence translation planes of order 32e . These planes appear to be new. Note also that only a few classes of ovoids ofQ(4,q) are known. Next we prove that each generalized quadrangle of order (q 2,q) arising from a flock of a quadratic cone has an ovoid. Finally, we give the following characterization of the classical generalized quadranglesQ(5,q): IfS is a generalized quadrangle of order (q,q 2),q even, having a subquadrangleS′ isomorphic toQ(4,q) and if inS′ each ovoid consisting of all points collinear with a given pointx ofS\S′ is an elliptic quadric, thenS is isomorphic toQ(5,q).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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