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  • 1
    Electronic Resource
    Electronic Resource
    Interspecific somatic hybrids between wild potato Solanum acaule Bitt. and anther-derived dihaploid potato (Solanum tuberosum L.) (1998)
    Springer
    Plant cell reports 18 (1998), S. 82-88 
    Details
    ISSN: 1432-203X
    Keywords: Key words DNA content ; Morphology ; Protoplast fusion ; RAPD ; Somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Solanum acaule Bitt. is a disomic tetraploid (4x) wild potato species which is resistant to several potato diseases. Introgression of disease resistance and abiotic stress tolerance to the tetrasomic tetraploid (4x) cultivated potato (S. tuberosum L.) gene pool via crossing has been limited due to the difference in the endosperm balance number. In the present study, protoplast fusion was applied to produce hexaploid (6x) somatic hybrids between the parental lines, tetraploid (4x) S. acaule and two anther-derived dihaploid (2x) lines of S. tuberosum cv. White Lady. One callus (0.4%) of a total of 229 calli obtained regenerated into shoots in the fusion combination S. acaule (+) White Lady 15.dh.8.2.2. All the regenerated shoots were confirmed to be interspecific somatic hybrids using species-specific RAPD markers. In another fusion combination, S. acaule (+) White Lady 7.dh.23.1.1, fifteen calli (5%) regenerated into a total of sixteen shoots from 289 calli. All the analysed somatic hybrids between S. acaule and S. tuberosum were hexaploid. The mean DNA content (2C value) of the combination S. acaule (+) White Lady 15.dh.8.2.2 somatic hybrids (4.55 pg), was approximately the sum (4.69 pg) of the DNA contents of the parental lines, S. acaule (2.95 pg) and S. tuberosum (1.74 pg). In the greenhouse, the two somatic hybrids analysed were normal in their morphological characteristics and more vigorous than their parental lines. Most of the morphological characteristics were closer to the tetraploid S. acaule than to the dihaploid S. tuberosum. The interspecific somatic hybrids are currently being tested for frost tolerance and glycoalkaloid composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050536
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  • 2
    Electronic Resource
    Electronic Resource
    A genetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 83-89 
    Details
    ISSN: 1432-2242
    Keywords: Key words Lens culinaris subsp. orientalis ; Recombinant inbred lines ; AFLP ; RAPD ; Genetic map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A genetic linkage map of Lens sp. was constructed with 177 markers (89 RAPD, 79 AFLP, six RFLP and three morphological markers) using 86 recombinant inbred lines (F6:8) obtained from a partially interspecific cross. The map covered 1073 cM of the lentil genome with an average distance of 6.0 cM between adjacent markers. Previously mapped RFLP markers were used as anchor probes. The morphological markers, pod indehiscence, seed-coat pattern and flower-color loci were mapped. Out of the total linked loci, 8.4% showed segregation distortion. More than one-fourth of the distorted loci were clustered in one linkage group. AFLP markers showed more segregation distortion than the RAPD markers. The AFLP and RAPD markers were intermingled and clustering of AFLPs was seldom observed. This is the most extensive genetic linkage map of lentil to-date. The marker density of this map could be used for the identification of markers linked to quantitative trait loci in this population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050869
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  • 3
    Electronic Resource
    Electronic Resource
    The use of Rapd markers for lentil genetic mapping and the evaluation of distorted F2 segregation (1997)
    Springer
    Euphytica 96 (1997), S. 405-412 
    Details
    ISSN: 1573-5060
    Keywords: Lens culinaris ; linkage ; RAPD ; segregation distortion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To maximize the extent of polymorphism within a mapping population wide crosses are often made, frequently resulting in distorted segregation. Two parents used in the crosses in this study contained ca 50% from wild lentil genome (Lens culinaris ssp. Orientalis). We investigated the use of random amplified polymorphic DNA (RAPD) in the lentil (Lens culinaris Med.), for genetic mapping and testing for segregation distortion in F2 populations. In cross 1, 83% of the RAPD markers showed segregation distortion, which was also observed for isozyme and morphological loci. By contrast, in cross 2, there was little (10%) segregation distortion. Out of 390 primers tested, 116 primers (29.7%) yielded 192 polymorphic fragments between parents of cross 2. This polymorphism was confirmed as reproducible. Seventy-eight segregating loci were analyzed for linkage, at a LOD score 〉 3.0, resulted in 28 RAPD, one RFLP, one morphological and three oligonucleotide markers, which were assigned to 9 linkage groups spanning 206 cM. Clearly, in lentil RAPD markers were valuable for genetic mapping and evaluation of segregation distortion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003045000568
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  • 4
    Electronic Resource
    Electronic Resource
    Variability of diffusive leaf resistance and transpiration rates of various potato genotypes and its possible contribution to water economy (1989)
    Springer
    Potato research 32 (1989), S. 275-282 
    Details
    ISSN: 1871-4528
    Keywords: S. tuberosum ; S. Phureja ; anther culture ; 4x−2x hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Diffusive leaf resistance and transpiration rate were assessed in various potato genotypes includingS. tuberosum cvs. Atlantic, Cara, Alpha and Désirée,S. phureja clone PP5, a hybrid of Atlantic X PP5, and two anther-derived tetraploids of PP5. Resistance and transpiration were assessed with an LI-1600 steady state porometer on well-watered plants and differences in leaf resistance and transpiration rate among genotypes were obtained. These results imply that water economy may be improved by genotypes exhibiting small water losses. Transpiration rates calculated by dividing the weight of water transpirted per second by the area of the plant's leaflets, were lower than transpiration rates of young expanding leaflets assessed with the porometer. Nevertheless, the relative differences among genotypes were evident and a similar relationship among genotypes was maintained in the two measures. Cv. Alpha had lower transpiration rates, compared to Cara and Désirée, corresponding with its greater stomatal resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357839
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