ISSN:
1573-4986
Keywords:
4-deoxy-NeuAc
;
glycoprotein
;
CMP-sialic acid synthase
;
sialyltransferase
;
sialidase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The sialic acid analogue,N-acetyl-4-deoxy-neuraminic acid, is readily activated by CMP-sialic acid synthase from bovine brain. We also show that sialyl-transfer from CMP-N-acetyl-4-deoxy-neuraminic acid to asialo-α 1-acid glycoprotein is achieved at a high rate using Galβ1-4GlcNAc α(2.6)-sialyltransferase from rat liver. In contrast toVibrio cholerae sialidase, fowl plague virus sialidase liberates boundN-acetyl-4-deoxy-neuraminic acid from the glycoprotein. Thus, as opposed to the general view, the action of neither synthase nor transferase depends on the presence of the hydroxy group at C-4 ofN-acetylneuraminic acid.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01049452
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