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  • Articles  (3)
  • bottle test  (2)
  • 3-phosphoglycerate  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Effects of phosphorus nutrition on the response of photosynthesis to CO2 and O2, activation of ribulose bisphosphate carboxylase and amounts of ribulose bisphosphate and 3-phosphoglycerate in spinach leaves (1988)
    Springer
    Photosynthesis research 15 (1988), S. 133-141 
    Details
    ISSN: 1573-5079
    Keywords: phosphorus nutrition ; photosynthesis ; ribulose bisphosphate carboxylase ; ribulose bisphosphate ; 3-phosphoglycerate ; O2 partial pressure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phosphorus-deficient spinach plants were grown by transferring them to nutrient solutions without PO4. Photosynthetic rates were measured at a range of intercellular CO2 partial pressures from 50–500 μbar and then the leaves were freeze-clamped in situ to measure ribulose bisphosphate carboxylase (Rubisco) activity and metabolite concentrations. Compared with control leaves, deficient leaves had significantly lower photosynthetic rates, percentage activation of Rubisco, and amounts of ribulose bisphosphate and 3-phosphoglycerate at all CO2 partial pressures. After feeding 10 mM PO4 to the petioles of detached deficient leaves, all these measurements increased within 2 hours. At atmospheric CO2 partial pressure the photosynthetic rate was stimulated in 19 mbar O2 compared with 200 mbar. At higher CO2 partial pressures this stimulation was less but the percentage stimulation in deficient leaves was no different from controls in either CO2 partial pressure. It was concluded that phosphorus deficiency affects both Rubisco activity and the capacity for ribulose bisphosphate regeneration, and possible causes are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035257
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  • 2
    Electronic Resource
    Electronic Resource
    Algal bioassays to determine toxicity of metal mixtures (1980)
    Springer
    Hydrobiologia 74 (1980), S. 199-208 
    Details
    ISSN: 1573-5117
    Keywords: metals ; toxicity ; algal assay ; bottle test ; algae ; Chlorella fusca ; Ankistrodesmus bibraianum ; Scenedesmus quadricauda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The shortcomings of many established toxicity criteria for metals have resulted from a simplification of bioassays performed with single metals. A more comprehensive approach was needed to diagnose the effects of metal mixtures on aquatic organisms. Using Chlorella, Ankistrodesmus and Scenedesmus as test organisms, we experimented on a number of factors which affected metal toxicity bioassays. These included metal interactions, algal competitions, species sensitivities, the ratio of an excess metal to other metals and nutrient levels. An alternative technique was finally established which involved an evaluation of individual assays of Chlorella and Ankistrodesmus in separate media and a tissue-metal analysis on Chlorella. Chlorella fusca, commonly found in lakes with high metal concentrations, showed high tolerance to mixed-metal solutions, while Ankistrodesmus proved to be very sensitive. By determining the maximum yield ratio between Ankistrodesmus and Chlorella (i.e. the A/Ch ratio) it was possible to compare the toxic strength of harmful metals according to an established standard curve of A/Ch ratio versus mixed-metal concentrations. The levels of tissue-metal analysed in Chlorella also gave some indication as to which metals were responsible for the toxicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00008753
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  • 3
    Electronic Resource
    Electronic Resource
    Metal interactions in algal toxicology: conventional versus in vivo tests (1981)
    Springer
    Hydrobiologia 85 (1981), S. 67-71 
    Details
    ISSN: 1573-5117
    Keywords: metal interactions ; toxicity ; algal assays ; bottle test ; algae ; Scenedesmus quadricauda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Different studies on the toxicity of the same metals to algae have often shown divergent and sometimes contradictory results. The inconsistency of these findings was often attributed to environmental factors such as the degree of chelation, complexation and precipitation of metals. Conventionally, toxicity tests of metal mixtures were conducted by adding metals to algae growing in synthetic media. In our study, we examined the ‘in vivo’ toxic effects of metals by pretreating the algal cells with one metal, resuspendeding them in fresh medium, then exposing them to a second metal. The algal response showed marked differences between the conventional and the new approach. The conventional approach shows that the toxicity depends upon the complexes formed externally, whereas the in vivo approach shows that the toxicity probably depends upon the molecular transformation of the metals internally.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011346
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