ISSN:
1573-4986
Keywords:
α1
;
3-Galactosyltransferase
;
branch specificity
;
oligo-(N-acetyllactosaminoglycans)
;
wheat germ agglutinin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract α1,3-Galactosylation of radiolabelled bi-antennary acceptors Galβ1-4GlcNAcβ1-3(Galβ1-4GlcNAcβ1-6)Gal-R (R=1-OH, β1-4GlcNAc or β1-4Glc) with bovine thymus α1,3-galactosyltransferase was studied. At all stages of the reactions the three acceptors reacted faster at the 1 → 6 linked arm than at the 1 → 3 linked branch. Hence, in addition to the doubly α1,3-galactosylated products, practically pure Galβ1-4GlcNAcβ1-3(Galα1-3Galβ1-4GlcNAcβ1-6)Gal-R could be obtained from the three acceptors in reactions that had proceeded to near completion. The isomeric mono-α1,3-galactosylated products were identified by using exoglycosidases to remove the branches unprotected by α1,3-galactoses and by subsequently identifying the resulting linear glycans chromatographically.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00731221
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