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  • Inorganic Chemistry  (6)
  • 23S rRNA  (3)
  • Agrobacterium tumefaciens  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Transformation of vitis tissue by different strains of Agrobacterium tumefaciens containing the T-6b gene (1992)
    Springer
    Plant cell reports 11 (1992), S. 192-195 
    Details
    ISSN: 1432-203X
    Keywords: Grapevine ; Agrobacterium tumefaciens ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Stem pieces and leaf disks of Vitis spp. were cocultured with Agrobacterium tumefaciens strains carrying the UidA (ß-glucuronidase = GUS) gene. The transformation efficiency was highly increased by using a modified T-6b gene (a gene from pTiTm4) which interferes with normal growth and allows regeneration of normal Nicotiana rustica plants (Tinland 1990). The strains first tested on stem segments were subsequently tested in a leaf explant system. On leaves the transformation efficiency of the strains was much lower than with stems. Both the T-6b gene and the hsp 70-T-6b gene (a modified T-6b gene under the control of a heat shock promoter) allowed the initiation of GUS-positive buds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232531
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  • 2
    Electronic Resource
    Electronic Resource
    Major differences between the rrnA operons of two strains of Agrobacterium vitis (1996)
    Springer
    Archives of microbiology 166 (1996), S. 68-70 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsAgrobacterium vitis ; Ribosomal RNA ; 16S ; rRNA ; 23S rRNA ; Bacterial phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sequence of the rrnA operon and its flanking regions was determined for the Agrobacterium vitis type strain NCPPB3554. Compared to the earlier obtained rrnA sequence of A. vitis strain S4, several important differences were noted: the sequences diverged at the 5′-flanking region, within the 16S–23S intergenic region, and within the 23S rRNA sequence. The B8 stem-loop structure at the 5′-end of the 23S rRNA of strain NCPPB3554 was 142 nt shorter than that of strain S4. These findings have important consequences for the use of ribosomal RNA gene sequences in phylogenetic comparisons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050357
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  • 3
    Electronic Resource
    Electronic Resource
    Sequence of the iaa and ipt region of different Agrobacterium tumefaciens biotype III octopine strains: reconstruction of octopine Ti plasmid evolution (1991)
    Springer
    Plant molecular biology 16 (1991), S. 601-614 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; Ti plasmid ; evolution ; IS elements ; host range
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The TA regions of biotype III octopine/cucumopine (OC) Ti plasmids are closely related to the TL region of the biotype I octopine Ti plasmids pTiAch5 and pTi15955. Sequence analysis shows that the limited and wide host range biotype III OC TA regions are derived from a common ancestor structure which lacked the 6a gene found in the biotype I octopine TL region. The TA region of the wide host range OC Ti plasmids has conserved most of the original TL-like structure. In most wide host range OC isolates the TA-iaaH gene is inactivated by the insertion of an IS866 element. However, the TA region of the wide host range isolate Hm1 carries an intact TA-iaaH gene. This gene encodes a biologically active product, as shown by root induction tests and indole-3-acetic acid measurements. The limited host range OC Ti plasmids pTiAB3 and pTiAg57 have shorter TA regions which are derived from a wide host range TA region. The AB3 type arose by an IS868-mediated, internal TA region deletion which removed the iaa genes and part of the ipt gene and left a copy of IS868 at the position of the deleted fragment. The pTiAB3 iaa/ipt deletion was followed by insertion of a second IS element, IS869, immediately 3′ of the ipt gene. pTiAg57 underwent the same iaa-ipt deletion as pTiAB3, but lacks the IS868 and IS869 elements. Analysis of the various TA region structures provides a detailed insight into the evolution of the biotype III OC strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023425
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  • 4
    Electronic Resource
    Electronic Resource
    Sequence of Agrobacterium tumefaciens biotype III auxin genes (1991)
    Springer
    Plant molecular biology 16 (1991), S. 733-738 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; iaa genes ; Ti plasmid evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023438
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  • 5
    Electronic Resource
    Electronic Resource
    Sequence and characterisation of a ribosomal RNA operon from Agrobacterium vitis (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 99-107 
    Details
    ISSN: 1617-4623
    Keywords: Key words Ribosomal genes ; Agrobacterium ; Evolution ; 23S rRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  One of the four ribosomal RNA operons (rrnA) from the Agrobacterium vitis vitopine strain S4 was sequenced. rrnA is most closely related to the rrn operons of Bradyrhizobium japonicum and Rhodobacter sphaeroides and carries an fMet-tRNA gene downstream of its 5S gene, as in the case of R. sphaeroides. The 16S rRNA sequence of S4 differs from the A. vitis K309 type strain sequence by only one nucleotide, in spite of the fact that S4 and K309 have very different Ti plasmids. The predicted secondary structure of the S4 23S rRNA shows several features that are specific for the alpha proteobacteria, and an unusual branched structure in the universal B8 stem. The 3′ ends of the three other rrn copies of S4 were also cloned and sequenced. Sequence comparison delimits the 3′ ends of the four repeats and defines two groups: rrnA/rrnB and rrnC/rrnD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174350
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  • 6
    Electronic Resource
    Electronic Resource
    Sequence and characterisation of a ribosomal RNA operon fromAgrobacterium vitis (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 99-107 
    Details
    ISSN: 1617-4623
    Keywords: Ribosomal genes ; Agrobacterium ; Evolution ; 23S rRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract One of the four ribosomal RNA operons (rrnA) from theAgrobacterium vitis vitopine strain S4 was sequenced.rrnA is most closely related to therrn operons ofBradyrhizobium japonicum andRhodobacter sphaeroides and carries an fMet-tRNA gene downstream of its 5S gene, as in the case ofR. sphaeroides. The 16S rRNA sequence of S4 differs from theA. vitis K309 type strain sequence by only one nucleotide, in spite of the fact that S4 and K309 have very different Ti plasmids. The predicted secondary structure of the S4 23S rRNA shows several features that are specific for the alpha proteobacteria, and an unusual branched structure in the universal B8 stem. The 3′ ends of the three otherrrn copies of S4 were also cloned and sequenced. Sequence comparison delimits the 3′ ends of the four repeats and defines two groups:rrnA/rrnB andrrnC/rrnD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174350
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  • 7
    Electronic Resource
    Electronic Resource
    A Structural Study of Selenobenzamides: Crystal Structures and Dynamic 13C NMR (1997)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 130 (1997), S. 49-54 
    Details
    ISSN: 0009-2940
    Keywords: 4-(p-Bromoselenobenzoyl)morpholine ; 4-[(p-(Dimethylamino)selenobenzoyl]morpholine ; Hindered rotation ; Hammett treatment ; Selenoamides ; Amides ; Selenium compounds ; Structure elucidation ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The solid-state structures of (p-bromoselenobenzoyl)morpholine (2a) and [p-(dimethylamino)selenobenzoyl]morpholine (2b) were determined by X-ray diffraction. Both molecules show a flat selenoamide group. The larger contribution of resonance stabilization by the aromatic ring carrying the p-dimethylamino substituent is reflected by the smaller interplanar angle Θ between the aromatic ring and the selenoamide group [53.3(1)° vs. 81.1(1)°] and by the shorter length of the C=Se bond [1.824(5) Å vs. 1.840(3) Å]. The Gibbs free energy of activation of C-N bond rotation (ΔG≠rot) of five p-substituted (selenobenzoyl)morpholines was determined by dynamic 13C NMR. The activation barriers were found to range from 61.6 kJ/mol (X = NNMe2) to 75.1 kJ/mol (X = H). The ΔG≠rot values of the corresponding (thiobenzoyl)morpholines were found to be from 3.2 kJ/mol (X = NMe2) to 5.0 kJ/mol (X = H) lower. In both cases, ΔG≠rot showed an excellent linear Hammett correlation with s̰+p.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19971300108
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  • 8
    Electronic Resource
    Electronic Resource
    Substitutionsreaktionen an Alkylbenzolen unter Verwendung von Trimethylsilylazid und Tetrafluor-1,2-ethandisulfenyl-dichlorid (1989)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 122 (1989), S. 1071-1072 
    Details
    ISSN: 0009-2940
    Keywords: 1,3,2-Dithiazolidines ; Staudinger reaction ; Sulfenyl halides ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Substitution reactions of Alkylbenzenes by Use of Trimethylsilyl Azide and Tetrafluoro-1,2-ethanedisulfenyl Dichloride1,3,2-Dithiazolidines 4 are obtained in moderate and good yields from various alkylbenzenes 1, trimethylsilyl azide (2), and tetrafluoro-1,2-ethanedisulfenyl dichloride (3). A radical mechanism is proposed for this reaction.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19891220610
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  • 9
    Electronic Resource
    Electronic Resource
    Darstellung von 1,2-Bisalkinylthioether des TetrafluorethansProfessor Ulrich Wannagat zum 65. Geburtstage am 31. Mai 1988 gewidmet (1988)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 560 (1988), S. 55-58 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Preparation of 1,2-Bisalkinylthioether of 1,1,2,2-Tetrafluoroethane1,2-Bisalkinylthioether of 1,1,2,2-tetrafluoroethane are obtained by known procedures with lithiumalkinylides, prepared in situ, and 1,1,2,2-tetrafluoroethane-1,2-bissulfenylchloride.The reactions proceed at low temperatures in non polar solvents. 2a-2e are obtained in acceptable yields. The compounds are air stable but moisture sensitive. The products have been characterized on the basis of n.m.r., i.r., and mass spectra.
    Notes: 1,2-Bisalkinylthioether des 1,1,2,2-Tetrafluorethans werden nach bekannten Verfahren mit in situ hergestellten Lithiumalkinyliden und 1,1,2,2-Tetrafluorethan-1,2,-bissulfenylchlorid 1 erhalten. Die Umsetzungen in unpolaren Lösungsmitteln bei tiefen Temperaturen ergeben die hydrolyseempfindlichen, luftstabilen Verbindungen 2a-2e. Die Produkte werden anhand von NMR-, IR- und Massenspektren charakterisiert.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19885600107
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  • 10
    Electronic Resource
    Electronic Resource
    High resolution electron microscopy studies of Ba4Nb14O23Dedicated to Professor Harald Schäfer on his 80th Birthday (1993)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 619 (1993), S. 133-137 
    Details
    ISSN: 0044-2313
    Keywords: Barium niobium oxide, Ba4Nb14O23 ; cluster ; electron microscopy ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Untersuchungen an Ba4Nb14O23 mittels hochauflösender Elektronenmikroskopie.Die neue Verbindung Ba4Nb14O23, wurde durch Tempern von Gemengen von Ba5Nb4O15, Nb2O5 und Nb bei 1 450°C unter Ar erhalten. Ba4Nb14O23 wurde durch Untersuchungen mittels hochauflösender Elektronenmikroskopie und Röntgenpulveruntersuchungen charakterisiert. Die Verbindung kristallisiert orthorhombisch C-zentriert mit a=20.782(4), b=12.448(3), c=4.147(1) Å und Z=2. Die Kristallstruktur von Ba4Nb14O23 kann als intergrowth zwischen BaNbO3 und NbO beschrieben werden. Charakteristische Baueinheiten sind Dreierketten spitzenverknüpfter Nb6-Oktaeder, die über Stränge perowskitartiger Baugruppen zu einem dreidimensionalen Netzwerk verknüpt sind.
    Notes: The new compound, Ba4Nb14O23, has been prepared by heating mixtures of Ba5Nb4O15, Nb2O5 and Nb at 1 450°C under Ar. Ba4Nb14O23 has been studied by means of high resolution electron microscopy and X-ray powder diffraction techniques. It has a C-centered orthorhombic unit cell with a=20.782(4), b=12.448(3), c=4.148(1) Å and Z=2. The structure of Ba4Nb14O23 can be considered as being an intergrowth between BaNbO3 and NbO. Characteristic building units are triple chains of corner sharing Nb6 octahedra which are connected via columns of the perovskite type structure to a three dimensional network.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19936190122
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