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  • 16S rRNA gene tags; Aluminium; Antimony; Area/locality; Arsenic; Barium; Cadmium; Caesium; Cerium; Cobalt; Copper; DEPTH, sediment/rock; Europium; Event label; geochemistry of porewaters; hydrothermal vent; ICP-OES; ICP-SF-MS, Thermo Scientific, Element XR; INDEX2016; INDEX2016_12ROV; INDEX2016_20ROV; Indian Ocean; Ion chromatography; Iron; Kairei field; Lanthanum; Lead; Manganese; metalliferous sediments; Molybdenum; Neodymium; Nickel; Nitrate; Pourquoi Pas ? (2005); Praseodymium; Samarium; Sample code/label; Silica, dissolved; Silver; Thallium; Tin; Tungsten; Uranium; Vanadium; VICTOR; Victor6000 ROV; Zinc  (1)
  • 207-1257C; 207-1258B; 207-1259C; 207-1261B; Acridine Orange Direct Counting (AODC); Archaea; Bacteria, abundance; Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH); DEPTH, sediment/rock; DRILL; Drilling/drill rig; DSDP/ODP/IODP sample designation; Event label; Joides Resolution; Leg207; Ocean Drilling Program; ODP; Prokaryotes, abundance as single cells; Sample code/label; Sample comment; South Atlantic Ocean; Walvis Ridge, Southeast Atlantic Ocean  (1)
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  • 1
    Publication Date: 2023-03-02
    Description: Deep-sea sediment samples were taken from the (wider) Kairei hydrothermal field area (25°S, 70°E) as well as a remote site (26°S, 71°E) in the Indian Ocean during the INDEX cruise 2016 with the N/O Pourquoi pas? (Ifremer, France). Push core samples from different areas of the Kairei vent field, as well as a sample from the remote site (~200 km south-east from the Kairei), were recovered with the help of the ROV VICTOR 6000 (Ifremer, France). All subsampling steps were carried out shipboard at 4 °C. Porewater from push cores was extracted with rhizons (CSS, 5 cm Rhizosphere Research Products B.V., Netherlands) at a resolution of 2-3 cm, fixed with 1% HNO3 for trace element analyses and stored at 4 °C. With sterile syringes (nozzles removed) 3 ml of 2 cm layers of sediment were transferred into sterile falcon tubes for DNA extraction and stored at –80 °C. Concentrations of minor and trace elements Li, Al, Rb, Cs, Sr, Ba, V, Mn, Fe, Co, Ni, Cu, Zn, Ag, Cd, Tl, Pb, In, Sn, Sb, Bi, W, Mo, U, Au, As, and L were determined by highresolution ICP-SF-MS (Element XR, Thermo Scientific) after 25-fold dilution and spiking with Y and Re for internal standardization using appropriate mass resolution settings.
    Keywords: 16S rRNA gene tags; Aluminium; Antimony; Area/locality; Arsenic; Barium; Cadmium; Caesium; Cerium; Cobalt; Copper; DEPTH, sediment/rock; Europium; Event label; geochemistry of porewaters; hydrothermal vent; ICP-OES; ICP-SF-MS, Thermo Scientific, Element XR; INDEX2016; INDEX2016_12ROV; INDEX2016_20ROV; Indian Ocean; Ion chromatography; Iron; Kairei field; Lanthanum; Lead; Manganese; metalliferous sediments; Molybdenum; Neodymium; Nickel; Nitrate; Pourquoi Pas ? (2005); Praseodymium; Samarium; Sample code/label; Silica, dissolved; Silver; Thallium; Tin; Tungsten; Uranium; Vanadium; VICTOR; Victor6000 ROV; Zinc
    Type: Dataset
    Format: text/tab-separated-values, 942 data points
    Location Call Number Expected Availability
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  • 2
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    PANGAEA
    In:  Supplement to: Schippers, Axel; Neretin, Lev N (2006): Data report: Microbiological AODC and CARD-FISH analysis of black shale samples from the Demerara Rise, ODP Leg 207. In: Mosher, DC; Erbacher, J; Malone, MJ (eds.) Proceedings of the Ocean Drilling Program, Scientific Results, College Station, TX (Ocean Drilling Program), 207, 1-6, https://doi.org/10.2973/odp.proc.sr.207.105.2006
    Publication Date: 2024-01-09
    Description: Subseafloor sediments harbor over half of all prokaryotic cells on Earth (Whitman et al., 1998). This immense number is calculated from numerous microscopic acridine orange direct counts (AODCs) conducted on sediment cores drilled during the Ocean Drilling Program (ODP) (Parkes et al., 1994, doi:10.1038/371410a0, 2000, doi:10.1007/PL00010971). Because these counts cannot differentiate between living and inactive or even dead cells (Kepner and Pratt, 1994; Morita, 1997), the population size of living microorganisms has recently been enumerated for ODP Leg 201 sediment samples from the equatorial Pacific and the Peru margin using ribosomal ribonucleic acid targeting catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) (Schippers et al., 2005, doi:10.1038/nature03302). A large fraction of the subseafloor prokaryotes were alive, even in very old (16 Ma) and deep (〉400 m) sediments. In this study, black shale samples from the Demerara Rise (Erbacher, Mosher, Malone, et al., 2004, doi:10.2973/odp.proc.ir.207.2004) were analyzed using AODC and CARD-FISH to find out if black shales also harbor microorganisms.
    Keywords: 207-1257C; 207-1258B; 207-1259C; 207-1261B; Acridine Orange Direct Counting (AODC); Archaea; Bacteria, abundance; Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH); DEPTH, sediment/rock; DRILL; Drilling/drill rig; DSDP/ODP/IODP sample designation; Event label; Joides Resolution; Leg207; Ocean Drilling Program; ODP; Prokaryotes, abundance as single cells; Sample code/label; Sample comment; South Atlantic Ocean; Walvis Ridge, Southeast Atlantic Ocean
    Type: Dataset
    Format: text/tab-separated-values, 90 data points
    Location Call Number Expected Availability
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