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  • 1
    ISSN: 1573-0867
    Keywords: biological nitrogen fixation ; control crops ; isotope dilution technique ; legumes ; 15N slow-release fertilizer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To apply the isotope dilution (ID) technique, it is necessary to grow the “N2-fixing” crop in a soil where the mineral N is labelled with15N. Normally the “N2-fixing” crop and a suitable non-N2-fixing control crop are grown in the same labelled soil and the15N enrichment of the control crop is assumed to be equal to the15N enrichment of the nitrogen (N) derived from the soil in the “N2-fixing” crop. In this case the proportion of unlabelled N being derived from the air via biological N2 fixation (BNF) in the “N2-fixing” crop will be proportional to the dilution of the enrichment of the N derived from the labelled soil. To label the soil, the technique most often used is to add a single addition of15N-labelled N fertilizer shortly before, at, or shortly after, the planting of the crops. Data in the literature clearly show that this technique results in a rapid fall in the15N enrichment of soil mineral N with time. Under these conditions, if the control and the “N2-fixing” crops have different patterns of N uptake from the soil they will inevitably obtain different15N enrichments in the soil-derived N. In this case the isotope dilution technique cannot be applied, or if it is, there will be an error introduced into, the estimate of the contribution of N derived from BNF. Several experiments are described which explore different strategies of application of the ID technique to attempt to attenuate the errors involved. The results suggest that it is wise to use slow-release forms of labelled N, or in some cases, multiple additions, to diminish temporal changes in the15N enrichment of soil mineral N. The use of several control crops produces a range of different estimates of the BNF contributions to the “N2-fixing” crops, and the extent of this range gives a measure of the accuracy of the estimates. Likewise the use of more than one15N enrichment technique in the same experiment will also give a range of estimates which can be treated similarly. The potential of other techniques, such as sequential harvesting of both control and test crops, are also discussed.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2021-12-15
    Description: Study of a natural analogue for risk assessment. Experimental and numerical modelling approach was applied. Technique validation for measuring the flux of a CO2 single point emission site. We did an experimental and numerical modelling approach to prove methods and results.
    Description: Carbon dioxide is an essential gas for life on earth although it can be lethal to living beings at high concentrations in the atmosphere. Episodic release of CO2 from underground can occur either from natural processes (i.e., mantle degassing, thermal decarbonation) or industrial (geological storage of CO2, CCS). CO2 is a colourless and odourless gas denser than air, and once released in the atmosphere from point sources, its dynamics is initially governed by buoyancy and a gas cloud can accumulate above the ground (gravitational phase) leading to the formation of the so-called “CO2 lakes”. With time, CO2 distribution is then governed by wind and atmospheric turbulence (passive dispersion phase). Natural analogues provide evidences of the impact of CO2 leakage on vegetal cover, wild life and human beings. In this work, the dynamics of CO2 in the atmosphere after ground emission is assessed to quantify their potential risk. Two approaches have been followed: (1) direct measurement of air concentration in a natural emission site, where formation of a “CO2 lake” is common and (2) numerical atmospheric modelling with the TWODEE code. The studied site is located in the Campo de Calatrava region in central Spain, which is known for a widespread degassing of mantle-derived CO2. This site, called Cañada Real, has a degassing rate between 1 and 3 tonnes of CO2 per day. When atmospheric conditions are quite stable, i.e., negligible wind speed, the formation of a blanket of CO2-enriched air is visible at naked eye reaching up to 50 cm high. The CO2 concentration measured in air is typically higher than 10,000 ppm in most monitoring stations. The measured data are consistent with the numerical models that predict maximum concentration between 40,000 and 70,000 ppm CO2 in air, which is by far higher than the 30,000 ppm threshold from which hazardous effects on human beings are observed. Conclusions from this work, however, indicate that the risk for humans even at large emission rates is low due to the CO2 dispersion effect into the atmosphere, and only under very particular conditions lethal effects are predicted.
    Description: Published
    Description: 38-47
    Description: 6V. Pericolosità vulcanica e contributi alla stima del rischio
    Description: 6A. Geochimica per l'ambiente e geologia medica
    Description: JCR Journal
    Keywords: Experimental and numerical modelling ; atmospheric dispersion ; Risk assessment ; Natural analogue ; Leakage ; Carbon storage ; carbon dioxide ; co2 field survey ; SOLID EARTH ; vulcanology ; geology
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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