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  • Chemistry  (18)
  • Quantum optics, physics of lasers, nonlinear optics, classical optics  (4)
  • Vacuoles  (4)
  • 15N  (3)
  • 1
    Publication Date: 2016-05-12
    Description: Author(s): Tom A. W. Wolterink, Ravitej Uppu, Georgios Ctistis, Willem L. Vos, Klaus-J. Boller, and Pepijn W. H. Pinkse We investigate two-photon quantum interference in an opaque scattering medium that intrinsically supports a large number of transmission channels. By adaptive spatial phase modulation of the incident wave fronts, the photons are directed at targeted speckle spots or output channels. From 10 3 experim… [Phys. Rev. A 93, 053817] Published Wed May 11, 2016
    Keywords: Quantum optics, physics of lasers, nonlinear optics, classical optics
    Print ISSN: 1050-2947
    Electronic ISSN: 1094-1622
    Topics: Physics
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  • 2
    Publication Date: 2012-08-17
    Description: Author(s): Carsten Cleff, Petra Groß, Carsten Fallnich, Herman L. Offerhaus, Jennifer L. Herek, Kai Kruse, Willem P. Beeker, Chris J. Lee, and Klaus-Jochen Boller We theoretically investigate ground-state depletion for subdiffraction-limited spatial resolution in coherent anti-Stokes Raman scattering (CARS) microscopy. We propose a scheme based on ground-state depopulation, which is achieved via a control laser light field incident prior to the CARS excitatio... [Phys. Rev. A 86, 023825] Published Thu Aug 16, 2012
    Keywords: Quantum optics, physics of lasers, nonlinear optics, classical optics
    Print ISSN: 1050-2947
    Electronic ISSN: 1094-1622
    Topics: Physics
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  • 3
    Publication Date: 2016-11-29
    Description: Author(s): T. Denis, M. W. van Dijk, J. H. H. Lee, R. van der Meer, A. Strooisma, P. J. M. van der Slot, W. L. Vos, and K.-J. Boller We demonstrate that photonic crystals can be used to generate powerful and highly coherent Cherenkov radiation that is excited by the injection of a beam of free electrons. Using theoretical and numerical investigations we present the startup dynamics and coherence properties of such a laser, in whi… [Phys. Rev. A 94, 053852] Published Mon Nov 28, 2016
    Keywords: Quantum optics, physics of lasers, nonlinear optics, classical optics
    Print ISSN: 1050-2947
    Electronic ISSN: 1094-1622
    Topics: Physics
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  • 4
    Publication Date: 2013-03-26
    Description: Author(s): Carsten Cleff, Petra Groß, Carsten Fallnich, Herman L. Offerhaus, Jennifer L. Herek, Kai Kruse, Willem P. Beeker, Chris J. Lee, and Klaus-Jochen Boller We present a theoretical investigation of stimulated emission pumping to achieve sub-diffraction-limited spatial resolution in coherent anti-Stokes Raman scattering (CARS) microscopy. A pair of control light fields is used to prepopulate the Raman state involved in the CARS process prior to the CARS... [Phys. Rev. A 87, 033830] Published Mon Mar 25, 2013
    Keywords: Quantum optics, physics of lasers, nonlinear optics, classical optics
    Print ISSN: 1050-2947
    Electronic ISSN: 1094-1622
    Topics: Physics
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 120 (1979), S. 31-34 
    ISSN: 1432-072X
    Keywords: Neurospora crassa ; Slime variant ; Vacuoles ; Isolation ; Hydrolases ; Arginine permease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method for the isolation of vacuoles based on polybase induced lysis of protoplasts of the cell wall deficient Neurospora crassa slime variant is described. Isolated vacuoles are characterized by 12 to 50 times increased specific activities of several hydrolases as compared with the total homogenate of protoplasts. Total α-amino nitrogen, arginine, and polyphosphate are also greatly enriched in these vacuoles. Vacuoles are equipped with a permease for the transport of basic amino acids across the tonoplast.
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  • 6
    ISSN: 1432-072X
    Keywords: Yeast ; Vacuoles ; Compartmentation ; Polyphosphate ; Arginine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolated and purified vacuoles from yeast protoplasts contain the bulk of the cellular pool of arginine. The arginine is firmly retained in the isolated vacuoles despite of the presence of a permease which mediates arginine diffusion through the vacuolar membrane (Boller et al., 1975). It is shown, mainly by equilibrium dialysis, on vacuolar extracts, that the retention of arginine in the vacuoles is due to binding by polyphosphate. The polyphosphate appears to be located exclusively in the vacuoles. Enzymes hydrolysing polyphosphate are also located in the vacuoles. Isolated vacuoles from arginine grown cells contain about three times as much polyphosphate as vacuoles from ammonium grown cells; the vacuolar pool of arginine is correspondingly greater. Thus there seems to be a close correlation between the storage of arginine and polyphosphate. This confirms the observation that under conditions provoking “polyphosphate overcompensation” (Liss and Langen, 1962) the accumulation of enormous quantities of polyphosphate is associated with that of corresponding quantities of arginine, provided this amino acid is supplied in the medium. Yet, under certain growth conditions the cells are able to store, and to mobilize, both arginine and polyphosphate independently.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 109 (1976), S. 115-118 
    ISSN: 1432-072X
    Keywords: Yeast ; Protoplasts ; Vacuoles ; Concanavalin A ; Membrane asymmetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolated vacuoles of Saccharomyces cerevisiae did not bind Concanavalin A (labelled with tritium or with a fluorescent dye) unless the vacuoles were rendered permeable and their inner membrane surface made accessible. Yeast protoplasts, on the other hand, bound large amounts of Concanavalin A on their surface, and the number of binding sites was not increased after a gentle lysis expected to expose also the inner surface of the plasmalemma. It is concluded that both the plasmalemma and the vacuolar membrane carry Concanavalin A binding sites exclusively on the surface opposite to the cytoplasmic matrix.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 105 (1975), S. 319-327 
    ISSN: 1432-072X
    Keywords: Yeast ; Spheroplasts ; Vacuoles ; Isolation ; Basic macromolecules ; Poly-dl-lysine ; DEAE-dextran ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The polybasic macromolecules DEAE-dextran (diethylaminoethyl-dextran, molecular weight 500 000) and poly-dl-lysine (molecular weight 30 000–70 000) were adsorbed with a high affinity by spheroplasts of Candida utilis and, subsequently, induced lysis. The extent of lysis of spheroplasts and of the liberated vacuoles was studied under various conditions using α-glucosidase activity and soluble arginine as cytoplasmic and vacuolar markers, respectively. Adsorption of polybases was rapidly completed even at 0°C; however, with small doses, lysis was poor at 0–12°C and extensive at temperatures above 12°C. This permitted the completion of adsorption before initiating lysis. The purified vacuoles were also sensitive to polybases though less so than the spheroplasts; however, after lysis of spheroplasts the liberated vacuoles were well protected against the action of polybases. A treatment with polybases which disrupted more than 99% of the spheroplasts left at least 70% of the vacuoles intact. Potassium chloride in high concentrations and calcium chloride in low concentrations inhibited polybase induced lysis of spheroplasts by preventing or even reversing the polybase adsorption. A polyacidic macromolecule, dextran sulfate, could prevent but not reverse the adsorption of polybase and subsequent lysis. Metabolic inhibitors reduced the susceptibility of spheroplasts to polybase induced lysis. Vacuoles isolated from polybase lysed spheroplasts still contained large pools of soluble amino acids, and their ability to transport arginine specifically is a further indication of their functional integrity.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 133 (1991), S. 47-56 
    ISSN: 1573-5036
    Keywords: clover ; competition ; grass ; Lolium perenne ; 15N ; nitrogen fixation ; Phleum pratense ; temperature ; Trifolium repens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract It was the aim of this study to determine the way in which low temperature modifies the effect of a competing grass on nitrogen fixation of a forage legume. White clover (Trifolium repens L.) was grown in monoculture or in different planting ratios with timothy (Phleum pratense L.) or perennial ryegress (Lolium perenne L.) in growth chambers at either 7.5/5°C (LoT) or 15/10°C (HiT) average day/night temperatures, and with 2.5 or 7.5 mM 15N-labelled nitrate in the nutrient solution. Competition with grass led to a marked increase in the proportion of clover nitrogen derived from symbiosis (% Nsym). This increase was slower at LoT where % Nsym was reduced considerably; it was closely related to the reduction in the amount of available nitrate as a result of its being utilized by the grass. Nitrogen concentration in white clover herbage and dry matter yield per clover plant were reduced, for the most part, when a competing grass was present. The amount of nitrogen fixed per plant of white clover decreased markedly with temperature. Low temperature consequently accentuated competition for nitrate. The capacity of white clover to compete successfully was limited by its slower growth and nitrogen accumulation.
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  • 10
    ISSN: 1573-5036
    Keywords: clover ; legume/grass mixture ; Lolium multiflorum ; Lolium perenne ; 15N ; nitrogen fixation ; nitrogen transfer ; Trifolium pratense ; Trifolium repens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field study was carried out near Zürich (Switzerland) to determine the yield of symbiotically fixed nitrogen (15N dilution) from white clover (Trifolium repens L.) grown with perennial ryegrass (Lolium perenne L) and from red clover (Trifolium pratense L.) grown with Italian ryegrass (Lolium multiflorum Lam.). A zero N fertilizer treatment was compared to a 30 kg N/ha per cut regime (90 to 150 kg ha−1 annually). The annual yield of clover N derived from symbiosis averaged 131 kg ha−1 (49 to 227 kg) without N fertilization and 83 kg ha−1 (21 to 173 kg) with 30 kg of fertilizer N ha−1 per cut in the seeding year. Values for the first production year were 308 kg ha−1 (268 to 373 kg) without N fertilization and 232 kg ha−1 (165 to 305 kg) with 30 kg fertilizer N ha−1 per cut. The variation between years was associated mainly with the proportion of clover in the mixtures. Apparent clover-to-grass transfer of fixed N contributed up to 52 kg N ha−1 per year (17 kg N ha−1 on average) to the N yield of the mixtures. Percentage N derived from symbiosis averaged 75% for white and 86% for red clover. These percentages were affected only slightly by supplemental nitrogen, but declined markedly during late summer for white clover. It is concluded that the annual yield of symbiotically fixed N from clover/grass mixtures can be very high, provided that the proportion of clover in the mixtures exceeds 50% of total dry mass yield.
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