ISSN:
1573-5001
Schlagwort(e):
absorptive lineshapes
;
13CO/13 $${\text{C}}^\alpha$$ decoupling
;
HNCO
;
sensitivity enhancement
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Abstract A simple scheme is presented for the suppression of dispersive contributions to cross peaks in HNCO-type spectra where the 15N chemical shift is recorded in a constant-time manner immediately prior to the transfer from 15 N to 1HN at the end of the sequence. These dispersive contributions arise when the delay for refocusing the 15N-13CO one-bond coupling is set to less than 0.5/1JN,CO and when 2 JHN,CO ≠ 0. Improvements in sensitivity in 1HN detected experiments recorded on 15 N,13C-labeled samples can be realized by application of 13CO/13 $${\text{C}}^\alpha$$ decoupling during acquisition. Sensitivity gains on the order of 15% and 5% have been obtained for an SH3 domain (62 residues) and maltose binding protein (370 residues), respectively.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1008381929212
Permalink
