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  • 1
    Electronic Resource
    Electronic Resource
    DNA binding and bending by a chloroplast-encoded HU-like protein overexpressed in Escherichia coli (1997)
    Springer
    Plant molecular biology 34 (1997), S. 339-343 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; DNA bending ; DNA binding ; HU-like protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the chloroplast HlpA protein also strongly facilitated cyclization of a short DNA fragment in the presence of T4 DNA ligase, indicating its ability to mediate very tight DNA curvatures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005867215258
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of the structure of lignin-carbohydrate complexes by the specific13C tracer method (2000)
    Springer
    Journal of wood science 46 (2000), S. 130-136 
    Details
    ISSN: 1611-4663
    Keywords: Lignin-carbohydrate complexes ; NMR ; 13C tracer ; Coniferin ; Ketal linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract In the present study the specifically13C-enriched lignin precursors of biosynthesis (i.e., coniferin-[side chainα−13C], coniferin-[side chain-β−13C] and coniferin-[side chain-γ−13C]) were synthesized and administered exogeneously to ginkgo shoots (Ginkgo biloba L.) to obtain13C-enriched lignin-carbohydrate complexes (LCCs). The specifically13C-enriched LCCs were isolated from the newly formed xylem of ginkgo shoots administered with the13C-enriched precursors and degraded by enzymes. Lignin-rich fractions, so called enzyme-degraded LCCs (EDLCCs), were obtained. By determining their13C-NMR spectra, information related to the chemical structure of lignin building units and linkages between phenylpropane units of lignin and carbohydrates were obtained. It was found that these precursors were incorporated in natural lignin successfully. Three lignin-carbohydrates linkages (i.e., ether type, ester type, ketal type) were found at the C α -position of the side chain of phenylpropane units in ginkgo LCC. No lignin-carbohydrate bond at the Cβ- or Cγ-position of the lignin side chain was observed in the13C-NMR spectra of the13C-enriched LCCs. This fact indicates that a specific13C tracer technique can be useful in NMR study of the chemical structure of LCCs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00777359
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  • 3
    Electronic Resource
    Electronic Resource
    A new method for measuring permeability of bio-impregnated textile grafts (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 1 (1990), S. 127-136 
    Details
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Traditional water permeability evaluation procedures developed for measurement of gross leakage rates of textile prostheses are not adequate for testing the permeability of low-porosity bio-impregnated vascular prostheses. Obtaining reliable water permeability data for these low porosity grafts requires a technique that can accurately assess minor water leakage under the applied pressure. This study proposes an automated method for graft permeability assessment which measures the pressure change inside the graft sample as a function of water loss. The new unit of water permeability defines the pressure gradient at the given initial conditions. The proposed method for evaluating the water permeability of bio-impregnated vascular prostheses provides highly reliable data, is accurate, and excludes human error.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jab.770010205
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  • 4
    Electronic Resource
    Electronic Resource
    A rapid in vitro test of the in vivo healing potential of vascular prostheses (1987)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 21 (1987), S. 1081-1091 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: An in vitro method for comparing the penetration of bovine fibroblasts seeded on the external surface of porous vascular prostheses was devised. The effects of water porosity reduction and differing manufacturing constructions (warp-knit Dacron, woven Dacron and polytetrafluoroethylene) on the ability of the bovine fibroblasts to penetrate transmurally was investigated. Of the warp-knit external-veloured Dacron prostheses, the highest porosity 140-denier prototype had the highest luminal surface cell count and the lowest porosty 280-denier prototype the lowest luminal surface cell count. The intermediate prototypes had values between these two extremes. The woven Dacron prostheses which were of even lower porosity but with a much thinner wall, had cell counts midway between the 140-denier and the 280-denier prototypes. The microporous polytetrafluoroethylene prostheses did not allow fibroblast penetration despite adherence of cells to the outer surface. These findings agree with in vivo healing studies of the same materials in the descending thoracic aorta of the dog, demonstrating that this rapid in vitro assay method can help predict the healing potential of a vascular prosthesis.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820210903
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