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Digitale Medien

Diversification of isoelectric points from isolated chloroplasts during the cell cycle of Euglena gracilis, strain z (1982)

Brandt, Peter ; Ernst, Dorothee

Weinheim : Wiley-Blackwell

Electrophoresis 3 (1982), S. 174-175

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ISSN: 0173-0835

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: We describe a rapid method whereby isolated chloroplasts from Euglena gracilis can be separated in a Ficoll gradient according to their surface pK's by isoelectric focusing in one hour or less. The linear Ficoll gradient is made isotonic over the whole separation distance by addition of sucrose. No released proteins of the isolated chloroplasts are detectable after isoelectric focusing. Modifications of the chloroplast envelope during the cell cycle of Euglena gracilis are evident by alterations of their isoelectric points.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/elps.1150030313

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Master track of METEOR cruise M181 in 1 sec resolution (zipped, 18.2 MB) (2022)

Brandt, Peter

PANGAEA

In: GEOMAR - Helmholtz Centre for Ocean Research Kiel

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Publikationsdatum: 2024-06-25

Beschreibung: Raw data acquired by position sensors on board RV METEOR during expedition M181 were processed to receive a validated master track which can be used as reference of further expedition data. During M181 the motion reference unit Kongsberg SeaTex AS MRU-5 combined with Kongsberg SeaTex AS Seapath 320 and two C and C Technologies GPS receivers C-NAV3050 were used as navigation sensors. Data were downloaded from DAVIS SHIP data base (https://dship.bsh.de) with a resolution of 1 sec. Processing and evaluation of the data is outlined in the data processing report. Processed data are provided as a master track with 1 sec resolution derived from the position sensors' data selected by priority and a generalized track with a reduced set of the most significant positions of the master track.

Schlagwort(e): 1 sec resolution; CT; DAM_Underway; DAM Underway Research Data; M181; M181-track; Meteor (1986); TRATLEQ 2; Underway cruise track measurements

Materialart: Dataset

Format: application/zip, 18.2 MBytes

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Physical oceanography from mooring KPO_1210 (2022)

Brandt, Peter ; Krahmann, Gerd

PANGAEA

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Publikationsdatum: 2024-06-25

Beschreibung: GEOMAR moorings are typically equipped with instruments recording pressure, temperature, conductivity, dissolved oxygen and current velocity. Instruments with pressure, temperature, conductivity and oxygen sensors were calibrated in situ immediately prior to and after a mooring deployment period by attaching them to the CTD frame during CTDO casts. Correction terms were then developed from the difference between the sensor readings and the calibrated CTDO data during several minute long calibration stops. These correction terms were then applied to the full deployment periods. This ensured best data quality with recognition of potential sensor drifts and also allowed for the estimation of calibration and measurement errors (Hahn et al. 2014, Bittig et al. 2018, Berx et al. 2019).

Schlagwort(e): Current velocity, east-west; Current velocity, north-south; DATE/TIME; DEPTH, water; Flag; KPO_1210; M158; M158_199-1; Meteor (1986); MOOR; Mooring; Oxygen; Pressure, water; Salinity; Sample code/label; SO284; SO284_71-1; Sonne_2; South Atlantic Ocean; Temperature, water

Materialart: Dataset

Format: text/tab-separated-values, 6397497 data points

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