ISSN:
1432-072X
Schlagwort(e):
Key words Heteroglycan
;
Cell walls
;
1
;
6-α-d-Mannan
;
Trichoderma
;
Aspergillus
;
β-Glucosidase
;
Glycosidases
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract A heteroglycan responsible for the binding of the enzyme β-1,4-d-glucosidase (EC 3.2.1.21) to fungal cell walls was isolated from cell walls of the filamentous fungus Trichoderma reesei. The heteroglycan, composed of mannose, galactose, glucose, and glucuronic acid, also activated β-1,4-d-glucosidase, β-1,4-d-xylosidase and N-acetyl-β-1,4-d-glucosaminidase activity in vitro. The structural backbone of this heteroglycan was prepared by acid hydrolysis and gel filtration. The molecular structure of the core of the heteroglycan was determined by NMR studies as a linear α-1,6-d-mannan. The mannan core obtained by acid degradation stimulated the β-glucosidase activity by 90%. Several glycosidases from Aspergillus niger were also activated by the T. reesei heteroglycan. The β-glucosidase of Trichoderma was activated by mannan from Saccharomyces cerevisiae to a comparable extent.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s002030050283
Permalink
