Publication Date:
2008-06-24
Description:
The heart is formed from cardiogenic progenitors expressing the transcription factors Nkx2-5 and Isl1 (refs 1 and 2). These multipotent progenitors give rise to cardiomyocyte, smooth muscle and endothelial cells, the major lineages of the mature heart. Here we identify a novel cardiogenic precursor marked by expression of the transcription factor Wt1 and located within the epicardium-an epithelial sheet overlying the heart. During normal murine heart development, a subset of these Wt1(+) precursors differentiated into fully functional cardiomyocytes. Wt1(+) proepicardial cells arose from progenitors that express Nkx2-5 and Isl1, suggesting that they share a developmental origin with multipotent Nkx2-5(+) and Isl1(+) progenitors. These results identify Wt1(+) epicardial cells as previously unrecognized cardiomyocyte progenitors, and lay the foundation for future efforts to harness the cardiogenic potential of these progenitors for cardiac regeneration and repair.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2574791/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2574791/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhou, Bin -- Ma, Qing -- Rajagopal, Satish -- Wu, Sean M -- Domian, Ibrahim -- Rivera-Feliciano, Jose -- Jiang, Dawei -- von Gise, Alexander -- Ikeda, Sadakatsu -- Chien, Kenneth R -- Pu, William T -- P50 HL074734/HL/NHLBI NIH HHS/ -- P50 HL074734-05/HL/NHLBI NIH HHS/ -- England -- Nature. 2008 Jul 3;454(7200):109-13. doi: 10.1038/nature07060. Epub 2008 Jun 22.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Harvard Stem Cell Institute and Department of Cardiology, Children's Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18568026" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Differentiation
;
*Cell Lineage
;
Gene Expression Regulation, Developmental
;
Green Fluorescent Proteins/genetics/metabolism
;
Heart/*embryology
;
Homeodomain Proteins/genetics/metabolism
;
Mice
;
Myocytes, Cardiac/*cytology/metabolism
;
Pericardium/*cytology/embryology/metabolism
;
Stem Cells/*cytology/metabolism
;
Transcription Factors/genetics/metabolism
;
WT1 Proteins/genetics/metabolism
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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