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  • 1
    Electronic Resource
    Electronic Resource
    Transposition of IS117 (the Streptomyces coelicolor A3(2) mini-circle) to and from a cloned target site and into secondary chromosomal sites (1990)
    Springer
    Molecular genetics and genomics 224 (1990), S. 65-71 
    Details
    ISSN: 1617-4623
    Keywords: Streptomyces ; Transposable element ; ϕC31 phage ; Gene replacement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary IS117, previously known as the 2.6 kb minicircle, is a transposable element found in Streptomyces coelicolor A3(2). It integrates predominantly into one preferred site when introduced into the closely related Streptomyces lividans 66, which lacks IS117. This preferred integration site was deleted from the S. lividans chromosome by replacement with an erythromycin resistance gene delivered by a ϕC31 phage vector. When IS117 was introduced into the resulting strain it integrated into many other sites, with some indication of site preference. By cloning a 200 by fragment centred on the preferred integration site onto a low copy number, self-transmissible Streptomyces plasmid derived from SCP2* it was shown that this sequence is sufficient to define the preferred site: IS117 integrates efficiently into this sequence from its preferred site in the host chromosome and at a lower frequency from the plasmid into the preferred site on the S. lividans chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259452
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin inStreptomyces coelicolor A3(2) (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 66-73 
    Details
    ISSN: 1617-4623
    Keywords: Streptomyces ; Polyketide antibiotic ; Mutational cloning ; ϕC31 phage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We determined the physical and transcriptional organisation of the set of previously cloned biosynthetic genes involved in the production of the polyketide antibiotic actinorhodin byStreptomyces coelicolor A3(2). Complementation and mutational cloning analyses (in part using new ϕC31 phage vectors incorporating a transcriptional terminator to block transcription from vector promoters into the cloned DNA) indicate that all the biosynthetic genes, including at least one regulatory (activator) gene, are clustered in a chromosomal region of about 26 kb. The genes are organised in at least four separate transcription units, ranging in size from 1 kb for the class III gene, to a polycistronic transcript of at least 5 kb for the class I, VII and IV genes. Indirect evidence shows that resistance to actinorhodin is also determined by the cloned DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02428033
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    Paper (German National Licenses)
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