Publication Date:
2015-10-03
Description:
Dendritic cells can capture and transfer retroviruses in vitro across synaptic cell-cell contacts to uninfected cells, a process called trans-infection. Whether trans-infection contributes to retroviral spread in vivo remains unknown. Here, we visualize how retroviruses disseminate in secondary lymphoid tissues of living mice. We demonstrate that murine leukemia virus (MLV) and human immunodeficiency virus (HIV) are first captured by sinus-lining macrophages. CD169/Siglec-1, an I-type lectin that recognizes gangliosides, captures the virus. MLV-laden macrophages then form long-lived synaptic contacts to trans-infect B-1 cells. Infected B-1 cells subsequently migrate into the lymph node to spread the infection through virological synapses. Robust infection in lymph nodes and spleen requires CD169, suggesting that a combination of fluid-based movement followed by CD169-dependent trans-infection can contribute to viral spread.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4651917/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4651917/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sewald, Xaver -- Ladinsky, Mark S -- Uchil, Pradeep D -- Beloor, Jagadish -- Pi, Ruoxi -- Herrmann, Christin -- Motamedi, Nasim -- Murooka, Thomas T -- Brehm, Michael A -- Greiner, Dale L -- Shultz, Leonard D -- Mempel, Thorsten R -- Bjorkman, Pamela J -- Kumar, Priti -- Mothes, Walther -- P01 AI078897/AI/NIAID NIH HHS/ -- P30 CA016359/CA/NCI NIH HHS/ -- P50 GM082545/GM/NIGMS NIH HHS/ -- P50GM082545/GM/NIGMS NIH HHS/ -- R01 AI097052/AI/NIAID NIH HHS/ -- R01 AI112443/AI/NIAID NIH HHS/ -- R01 CA098727/CA/NCI NIH HHS/ -- R01 DA036298/DA/NIDA NIH HHS/ -- S10 RR026697/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2015 Oct 30;350(6260):563-7. doi: 10.1126/science.aab2749. Epub 2015 Oct 1.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06510, USA. sewald@mvp.uni-muenchen.de priti.kumar@yale.edu walther.mothes@yale.edu. ; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. ; Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06510, USA. ; Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510, USA. ; Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA. ; Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA. ; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA. ; The Jackson Laboratory, Bar Harbor, ME 04609, USA. ; Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510, USA. sewald@mvp.uni-muenchen.de priti.kumar@yale.edu walther.mothes@yale.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26429886" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Dendritic Cells/immunology/virology
;
HIV Infections/*immunology
;
HIV-1/*physiology
;
Humans
;
Leukemia Virus, Murine/*physiology
;
Lymph Nodes/immunology/virology
;
Lymphocytes/immunology/*virology
;
Macrophages/immunology/virology
;
Mice
;
Mice, Inbred C57BL
;
Mice, Knockout
;
Retroviridae Infections/*immunology
;
Sialic Acid Binding Ig-like Lectin 1/genetics/*physiology
;
Spleen/immunology/virology
;
*Virus Internalization
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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