Publication Date:
1998-12-04
Description:
The dependence of mitosis on the completion of the period of DNA replication in the cell cycle [synthesis (S) phase] ensures that chromosome segregation occurs only after the genome has been fully duplicated. A key negative regulator of mitosis, the protein kinase Wee1, was degraded in a Cdc34-dependent fashion in Xenopus egg extracts. This proteolysis event was required for a timely entrance into mitosis and was inhibited when DNA replication was blocked. Therefore, the DNA replication checkpoint can prevent mitosis by suppressing the proteolysis of Wee1 during S phase.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Michael, W M -- Newport, J -- R01GM44656/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1998 Dec 4;282(5395):1886-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, University of California, San Diego, La Jolla, CA 92093-0347, USA. wmichael@biomail.ucsd.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9836638" target="_blank"〉PubMed〈/a〉
Keywords:
Anaphase-Promoting Complex-Cyclosome
;
Animals
;
Aphidicolin/pharmacology
;
Cell Cycle Proteins/metabolism
;
Cell Nucleus/metabolism
;
Cyclin-Dependent Kinase Inhibitor p27
;
DNA Replication/drug effects
;
Female
;
G2 Phase
;
Leupeptins/pharmacology
;
Ligases/*metabolism
;
Male
;
Maturation-Promoting Factor/metabolism
;
Microtubule-Associated Proteins/metabolism
;
*Mitosis
;
*Nuclear Proteins
;
Okadaic Acid/pharmacology
;
Ovum
;
Phosphoprotein Phosphatases/metabolism
;
Protein-Serine-Threonine Kinases/metabolism
;
Protein-Tyrosine Kinases/*metabolism
;
Proteins
;
Recombinant Proteins/metabolism
;
*S Phase
;
Spermatozoa
;
*Tumor Suppressor Proteins
;
*Ubiquitin-Protein Ligase Complexes
;
Ubiquitin-Protein Ligases
;
Ubiquitins/analogs & derivatives/metabolism/pharmacology
;
Xenopus
;
*Xenopus Proteins
;
cdc25 Phosphatases
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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