Publication Date:
1991-11-22
Description:
Intrinsic transcription terminators of prokaryotes are distinguished by a common RNA motif: a stem-loop structure high in guanine and cytosine content, followed by multiple uridine residues. Models explaining intrinsic terminators postulate that the stem-loop sequence is necessary only to form structure. In the tR2 terminator of coliphage lambda, single-nucleotide changes reducing potential RNA stem stability eliminated tR2 activity, and a compensatory change that restored the stem structure restored terminator activity. However, multiple changes in the stem sequence that should have either maintained or increased stability reduced terminator activity. These results suggest that the ability of the stem-loop structure to signal transcription termination depends on sequence specificity and secondary structure.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cheng, S W -- Lynch, E C -- Leason, K R -- Court, D L -- Shapiro, B A -- Friedman, D I -- AI1459-10/AI/NIAID NIH HHS/ -- N01-CO-74101/CO/NCI NIH HHS/ -- New York, N.Y. -- Science. 1991 Nov 22;254(5035):1205-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1835546" target="_blank"〉PubMed〈/a〉
Keywords:
Bacteriophage lambda/*genetics
;
Base Sequence
;
DNA Mutational Analysis
;
Gene Expression Regulation, Viral
;
Genes, Viral
;
Molecular Sequence Data
;
Nucleic Acid Conformation
;
RNA, Messenger/genetics
;
RNA, Viral/genetics
;
*Regulatory Sequences, Nucleic Acid
;
Restriction Mapping
;
*Terminator Regions, Genetic
;
*Transcription, Genetic
;
Viral Structural Proteins/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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