Publication Date:
2002-11-16
Description:
Chromatin remodeling is required for efficient transcription of eukaryotic genes. In a genetic selection for budding yeast mutants that were defective in induction of the phosphate-responsive PHO5 gene, we identified mutations in ARG82/IPK2, which encodes a nuclear inositol polyphosphate kinase. In arg82 mutant strains, remodeling of PHO5 promoter chromatin is impaired, and the adenosine triphosphate-dependent chromatin-remodeling complexes SWI/SNF and INO80 are not efficiently recruited to phosphate-responsive promoters. These results suggest a role for the small molecule inositol polyphosphate in the regulation of chromatin remodeling and transcription.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458531/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1458531/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Steger, David J -- Haswell, Elizabeth S -- Miller, Aimee L -- Wente, Susan R -- O'Shea, Erin K -- GM51219/GM/NIGMS NIH HHS/ -- GM51377/GM/NIGMS NIH HHS/ -- R01 GM051377/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2003 Jan 3;299(5603):114-6. Epub 2002 Nov 14.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0448, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12434012" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphatases/metabolism
;
Chromatin/*metabolism
;
DNA-Binding Proteins/metabolism
;
Fungal Proteins/genetics/metabolism
;
Gene Expression Regulation, Fungal
;
Inositol Phosphates/*metabolism
;
Mutation
;
*Nuclear Proteins
;
Phosphate Transport Proteins/*genetics/metabolism
;
Phosphotransferases (Alcohol Group Acceptor)/genetics/metabolism
;
Point Mutation
;
Promoter Regions, Genetic
;
Protein Binding
;
Proton-Phosphate Symporters/genetics/metabolism
;
*Saccharomyces cerevisiae Proteins
;
Saccharomycetales/genetics/*metabolism
;
Transcription Factors/metabolism
;
*Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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