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  • Life and Medical Sciences  (4)
  • photosynthesis  (2)
  • *Protein Engineering  (1)
  • 1
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    Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins (2001)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2001-09-05
    Description: We report a flexible strategy for transducing ligand-binding events into electrochemical responses for a wide variety of proteins. The method exploits ligand-mediated hinge-bending motions, intrinsic to the bacterial periplasmic binding protein superfamily, to establish allosterically controlled interactions between electrode surfaces and redox-active, Ru(II)-labeled proteins. This approach allows the development of protein-based bioelectronic interfaces that respond to a diverse set of analytes. Families of these interfaces can be generated either by exploiting natural binding diversity within the superfamily or by reengineering the specificity of individual proteins. These proteins may have numerous medical, environmental, and defense applications.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Benson, D E -- Conrad, D W -- de Lorimier, R M -- Trammell, S A -- Hellinga, H W -- New York, N.Y. -- Science. 2001 Aug 31;293(5535):1641-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Box 3711, Duke University Medical Center, Durham, NC 27710, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11533486" target="_blank"〉PubMed〈/a〉
    Keywords: Allosteric Regulation ; Allosteric Site ; Animals ; Beer ; *Biosensing Techniques ; Blood Glucose/analysis ; Carrier Proteins/*chemistry/genetics/*metabolism ; Electrochemistry ; Electrodes ; Ligands ; Maltose/analysis ; Maltose-Binding Proteins ; Monosaccharide Transport Proteins/chemistry/metabolism ; Mutation ; Oxidation-Reduction ; Protein Conformation ; *Protein Engineering ; Rats ; *Ruthenium ; Signal Transduction ; Thermodynamics ; Zinc/chemistry/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
    Electronic Resource
    Electronic Resource
    Inhibition by phosphate of light-state transitions in cyanobacterial cells (1993)
    Springer
    Photosynthesis research 38 (1993), S. 135-140 
    Details
    ISSN: 1573-5079
    Keywords: photosynthesis ; light-harvesting ; light-state transition ; signal transduction ; cyanobacteria ; Synechococcus 6301 ; Synechococcus sp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Light-state transitions in cyanobacteria are a rapid physiological adaptation mechanism which changes the distribution of excitation energy absorbed by the light-harvesting complexes between Photosystem II and Photosystem I. State transitions in two cyanobacterial species are shown to be inhibited by buffers containing 0.2–0.4 M phosphate. Both the state 1 and the state 2 transition are inhibited, so that cells may be locked in the state to which they were adapted before the addition of phosphate. The inhibition of the state 1 transition is due to inhibition of photosynthetic electron transport. However, the inhibition of the state 2 transition is probably due to a direct effect on the biochemical signal transduction pathway. The implications for the biochemical mechanism of state transitions are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00146412
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  • 3
    Electronic Resource
    Electronic Resource
    State 1-State 2 transitions in the cyanobacterium Synechococcus 6301 are controlled by the redox state of electron carriers between Photosystems I and II (1990)
    Springer
    Photosynthesis research 23 (1990), S. 297-311 
    Details
    ISSN: 1573-5079
    Keywords: Cyanobacteria (Synechococcus 6301) ; excitation energy distribution ; light harvesting ; photosynthesis ; state transition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mechanism by which state 1-state 2 transitions in the cyanobacterium Synechococcus 6301 are controlled was investigated by examining the effects of a variety of chemical and illumination treatments which modify the redox state of the plastoquinone pool. The extent to which these treatments modify excitation energy distribution was determined by 77K fluorescence emission spectroscopy. It was found that treatment which lead to the oxidation of the plastoquinone pool induce a shift towards state 1 whereas treatments which lead to the reduction of the plastoquinone pool induce a shift towards state 2. We therefore propose that state transitions in cyanobacteria are triggered by changes in the redox state of plastoquinone or a closely associated electron carrier. Alternative proposals have included control by the extent of cyclic electron transport around PS I and control by localised electrochemical gradients around PS I and PS II. Neither of these proposals is consistent with the results reported here.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034860
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  • 4
    Electronic Resource
    Electronic Resource
    Structure and carbohydrate histochemistry of the stomach in eight species of teleosts (1978)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 158 (1978), S. 155-167 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The histology and carbohydrate histochemistry of eight teleostean stomachs are compared. Three gross anatomical types of stomachs are described and their shapes appear to correlate somewhat with feeding habits. Each type can be divided histologically into a corpus and pylorus. Gastric glands, containing only one cell type, occur in the copora of all species, but are present in the pylori of esocids only. As a single cell can produce both enzymes and hydrochloric acid such cells may be comparable to those of amphibians but not mammals. Lamina propria and submucosa are indistinctly separated in corpora but better defined in pylori by an intervening muscularis mucosa. The arrangement of the muscularis into inner circular and outer longitudinal layers is the opposite of that seen in the esophagus. Gastric mucous cells show species variations in localization of epithelial mucosubstances, which in broad terms are recognized as sulfomucins, sialomucins and neutral mucosubstances. A piscivorous diet does not appear to demand any particular type of carbohydrate. Within the Centrarchidae, gastric pit cells vary in carbohydrate content from only neutral mucosubstance to only weakly acidic sulfomucin; two species contain both types. A positive PAS reaction on the surface of gastric epithelial cells is suggestive of a striated border and thus possibly absorptive function. The absence of stomachs in some teleosts and the evolutionary and dietary significances are discussed.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051580203
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  • 5
    Electronic Resource
    Electronic Resource
    Structure and carbohydrate histochemistry of the esophagus in ten teleostean species (1977)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 152 (1977), S. 303-313 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The histology and carbohydrate histochemistry of ten teleostean esophagi were compared. Structurally, the four layers of a typical vertebrate digestive tract were consistently present. The epithelium was always stratified and in all but one species (Ictalurus nebulosus) contained taste buds. Esophageal mucous cells were not the typical goblet cells seen in other vertebrates but appeared to be of six different types, pairs of which were associated with particular families. In esocids, poorly developed mucous acini and serous monogranular cells were present. In all species, the subepithelial connective tissue was not divided into definitive lamina propriae and submucosae due to the absence of muscularis mucosae. Variably present in this connective tissue region were argentophilic fibers and in esocids only, randomly dispersed striated muscle fibers. The arrangement of the muscularis was reverse to that of the general vertebrate plan. In mucous cells, three general types of epithelial mucosubstances were identified and in broad terms were recognized as sulfomucins, sialomucins and neutral mucosubstances. Morphological differences were accompanied by differences in carbohydrate localization, each esophageal epithelium containing at least two different mucosubstances. However, the mucosubstances identified in each mucous cell had a profile of characteristics different in some respects from any other. Thus teleostean esophagi appear to perform an integrated diversity of functions as reflected by their complex morphology and carbohydrate histochemistry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051520303
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  • 6
    Electronic Resource
    Electronic Resource
    Structure and carbohydrate histochemistry of the intestine in ten teleostean species (1979)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 162 (1979), S. 343-359 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The morphology and carbohydrate histochemistry of ten teleostean intestines are compared. Although there is an absence of regional differentiation seen in higher vertebrates, specializations in some species occur in the form of intestinal swellings, pyloric ceca and recta, the latter separated by a valve. The intestinal lumen is lined by a simple columnar epithelium interspersed with goblet cells; multicellular intestinal glands are absent. Thick basement membranes seen in centrarchids and Perca flavescens closely resemble the stratum compactum found in the lamina propria of esocids. Granular cells, which vary in number from species to species, are often seen in the mucosa and submucosa but less frequently in the muscularis. In species with intestino-rectal valves, a rectum is easily defined by the abrupt appearance of lower mucosal folds, more goblet cells and a thicker muscularis. In the remaining species the above features appear gradually in the distal intestine. Goblet cells show species variations in localization of epithelial mucosubstances, which in broad terms are recognized as sulfomucins, sialomucins and neutral mucosubstances. In both proximal and distal intestines the majority of goblet cells contain sialomucin although small amounts of sulfomucin are also often present. In species without intestino-rectal valves, no changes in carbohydrates occur between proximal and distal intestines. The possible significance of the heterogeneous character of digestive tract mucosubstances is discussed.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051620305
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  • 7
    Electronic Resource
    Electronic Resource
    Genes differentially expressed with malignant transformation and metastatic tumor progression of murine squamous cell carcinoma (1997)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 67 (1997), S. 90-100 
    Details
    ISSN: 0730-2312
    Keywords: squamous cell carcinoma ; differential display ; tropomyosin α ; cytokine KC ; ribosomal protein L18a ; antigen Sp17 ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Molecular changes occurring with tumor formation and metastasis need to be identified in order to define novel markers and targets for chemoprevention and therapy. Cell lines from a multistage model of murine squamous cell carcinoma were analyzed for differences in gene expression using mRNA differential display. mRNA was isolated from primary keratinocytes, an in vitro transformed keratinocyte line (Pam 212), and three metastatic cell lines derived from Pam 212 following tumor progression in vivo. cDNA was synthesized by reverse transcription and amplified by PCR using 72 primer combinations to screen and compare approximately 3,600 sequences. Five cDNAs with a differential expression pattern confirmed by Northern blot analysis were cloned and sequenced, revealing homology with known genes. The gene encoding tropomyosin α was preferentially expressed in primary keratinocytes; genes for tyrosine kinase Yes-associated protein (YAP65) and ribosomal protein L18a were preferentially expressed in transformed and metastatic tumor cell lines; and genes for the Gro-α family cytokine KC and antigen Sp17 exhibited increased expression in the three metastatic cell lines. The structure and function of the genes identified suggest that they may possibly be linked to cell shape and motility, signal transduction, protein synthesis, growth, granulocyte chemotaxis, and angiogenesis. This study demonstrates the ability of mRNA differential display to detect altered gene expression in this tumor progression model of murine squamous cell carcinoma, and the potential usefulness of this approach for identification of candidate genes as chemoprevention markers and targets. J. Cell. Biochem. Suppls. 28/29:90-100. Published 1998 Wiley-Liss, Inc.This article is a US Government work and, as such, is in the public domain in the United States of America.
    Additional Material: 3 Ill.
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