Publication Date:
2001-08-04
Description:
The development of resistance is the main threat to the long-term use of toxins from Bacillus thuringiensis (Bt) in transgenic plants. Here we report the cloning of a Bt toxin resistance gene, Caenorhabditis elegans bre-5, which encodes a putative beta-1,3-galactosyltransferase. Lack of bre-5 in the intestine led to resistance to the Bt toxin Cry5B. Wild-type but not bre-5 mutant animals were found to uptake toxin into their gut cells, consistent with bre-5 mutants lacking toxin-binding sites on their apical gut. bre-5 mutants displayed resistance to Cry14A, a Bt toxin lethal to both nematodes and insects; this indicates that resistance by loss of carbohydrate modification is relevant to multiple Bt toxins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Griffitts, J S -- Whitacre, J L -- Stevens, D E -- Aroian, R V -- New York, N.Y. -- Science. 2001 Aug 3;293(5531):860-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11486087" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Bacterial Proteins/metabolism/*toxicity
;
*Bacterial Toxins
;
Biological Transport
;
Caenorhabditis elegans/enzymology/*genetics/metabolism
;
*Caenorhabditis elegans Proteins
;
Cloning, Molecular
;
Digestive System/enzymology/metabolism
;
Disorders of Sex Development
;
Drug Resistance/genetics
;
Endocytosis
;
Endotoxins/metabolism/*toxicity
;
Feeding Behavior
;
Galactosyltransferases/chemistry/*genetics/*metabolism
;
Genes, Helminth
;
Hemolysin Proteins
;
*Insect Proteins
;
Molecular Sequence Data
;
Mosaicism
;
Mutation
;
*Pest Control, Biological
;
Receptors, Cell Surface/metabolism
;
Transformation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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