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  • ATOMIC AND MOLECULAR PHYSICS  (8)
  • *N-Acetylgalactosaminyltransferases  (1)
  • 1
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    American Association for the Advancement of Science (AAAS)
    Publikationsdatum: 1988-11-11
    Beschreibung: The glycoprotein hormones lutropin (LH) and follitropin (FSH), which have common alpha-subunits but hormone-specific beta-subunits, are both synthesized in the gonadotroph. However, they bear Asn-linked oligosaccharides that differ in structure. Those on LH terminate with the sequence SO4-4GalNAc beta 1----4GlcNAc beta 1----2Man alpha, whereas those on FSH terminate with the sequence sialic acid alpha-Gal beta 1----4GlcNAc beta 1----2Man alpha. A GalNAc-transferase was identified in bovine pituitary membranes that recognizes features of the alpha-subunit peptide and adds GalNAc to its oligosaccharides with an apparent Michaelis constant of 25 micromolar. The different patterns of glycosylation for LH and FSH indicate that access to the protein recognition marker on the alpha-subunit is modulated by the associated beta-subunit. The tightly regulated synthesis of sulfated and sialylated oligosaccharides on the pituitary glycoprotein hormones suggests these oligosaccharides have an important biological role.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Smith, P L -- Baenziger, J U -- HD-20197/HD/NICHD NIH HHS/ -- R37-CA21923/CA/NCI NIH HHS/ -- T32-ES07066/ES/NIEHS NIH HHS/ -- New York, N.Y. -- Science. 1988 Nov 11;242(4880):930-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2460923" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acetylgalactosamine/metabolism ; Animals ; Carbohydrate Sequence ; Cattle ; Cell Membrane/enzymology ; Follicle Stimulating Hormone/*biosynthesis/metabolism ; Follicle Stimulating Hormone, beta Subunit ; Galactosyltransferases/*metabolism ; Glycoprotein Hormones, alpha Subunit/metabolism ; Glycosylation ; Humans ; Luteinizing Hormone/*biosynthesis/metabolism ; Molecular Sequence Data ; *N-Acetylgalactosaminyltransferases ; Oligosaccharides/metabolism ; Pituitary Gland/*enzymology ; Placenta/enzymology ; Sulfates/metabolism
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
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  • 2
    Publikationsdatum: 2019-06-27
    Beschreibung: The paper introduces a procedure that combines measurements of absorption and emission by atoms to obtain relative oscillator strengths that are independent of temperature determination in the sources and of assumptions regarding local thermodynamic equilibrium. The experimental observations are formed into sets of transitions and required to satisfy defined ratios. The procedure is illustrated with the published data of Whaling et al. and Smith and Kuehne for 16 transitions in Ti I. It is shown that the relative oscillator strengths resulting from this procedure have calculated uncertainties between 5 and 17% (about 95% confidence level). Evidence is presented to suggest that these uncertainties have been overestimated.
    Schlagwort(e): ATOMIC AND MOLECULAR PHYSICS
    Materialart: Physical Review A - General Physics; vol. 20
    Format: text
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  • 3
    Publikationsdatum: 2019-06-28
    Beschreibung: A new technique is presented for the measurement of transition probabilities for weak allowed, intersystem, and forbidden lines. The method exploits the fact that oscillator strength is proportional to the number of stimulated absorptions and emissions produced by a narrow-band laser pulse of known energy which is in resonance with an atomic transition. The method is tested for a particular transition of Mg I with a known oscillator strength value and of appropriate magnitude. The number densities are measured using a Mach-Zehnder interferometer and the hook method for the lower level population and by measuring an absorption-equivalent width for the other. The apparatus consisted of a high-power tunable laser and a magnesium oven to produce excited Mg vapor, and a laser-plasma background continuum. The results are in good agreement with theoretical and other experimental data.
    Schlagwort(e): ATOMIC AND MOLECULAR PHYSICS
    Materialart: Physical Review A - General Physics; vol. 25
    Format: text
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  • 4
    Publikationsdatum: 2019-06-27
    Beschreibung: Oscillator strengths for 134 lines from the ground term of neutral titanium have been measured by the hook method. The data, which are normalized to those of Bell, Kalman and Tubbs, agree well with most previously measured data but indicate that there is a line strength dependent error in the National Bureau of Standards compilation of TiIf-values by Wiese and Fuhr.
    Schlagwort(e): ATOMIC AND MOLECULAR PHYSICS
    Format: text
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  • 5
    Publikationsdatum: 2019-06-28
    Beschreibung: The radiative lifetime of the 5S(0)2 metastable level of O(2+) was measured as 1.22 + or - 0.08 ms at the 90 percent confidence level by observing the time dependence of the spontaneous emission from metastable ions created and stored in a cylindrical radio-frequency ion trap. The intersystem line emission 2s(2)2p(2) 3P - 2s2p(3) 5S(0) was observed at 1660.8 and 1666.2 A. Discrepancies between measured and calculated values indicate that certain calculated transition probabilities for intersystem lines may be less reliable than previously believed.
    Schlagwort(e): ATOMIC AND MOLECULAR PHYSICS
    Materialart: Astrophysical Journal, Part 1 (ISSN 0004-637X); 281; 477-481
    Format: text
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  • 6
    Publikationsdatum: 2019-06-27
    Beschreibung: A source for the continuous production of neutral metastable atoms is described. A hollow cathode discharge tube was used to produce densities of 3.5 times 10 to the 13th per cu cm and column densities of 1.6 times 10 to the 15th per sq cm in the 4s(2)2D metastable term of neutral copper. The accuracy of these density determinations (+ or - 30 to + or - 66%) is limited by the oscillator strengths required for the analysis. The usefulness of the source was demonstrated by measurements of relative oscillator strengths of some Cu I lines in the 2200 A wavelength region. These data are compared with those obtained by other methods.
    Schlagwort(e): ATOMIC AND MOLECULAR PHYSICS
    Materialart: Optical Society of America; vol. 69
    Format: text
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  • 7
    Publikationsdatum: 2019-08-28
    Beschreibung: Photoabsorption cross sections for five CO bands, at wavelengths between 96.7 and 98.8 nm, have been measured at high-resolution in a supersonic jet-cooled source at the Photon Factory synchrotron facility. New integrated cross sections are reported for the K-X, L(prime)-X, and L-X bands. Low-temperature spectra of the J-X and W-X bands, which were used in the determination of the absorbing CO column densities, are also presented. The rotational structures of the K-X, L(prime)-X, and L-X bands do not overlap in the low-temperature spectra, allowing for the first unambiguous determination of these band oscillator strengths. We also report revised room temperature measurements of integrated cross sections for the K-X, L(prime)-X, and L-X bands, in which distortions in the measured spectra due to insufficient instrumental resolution have been minimized; the revised room temperature integrated cross sections are consistent with the low-temperature results.
    Schlagwort(e): ATOMIC AND MOLECULAR PHYSICS
    Materialart: Astrophysical Journal, Part 1 (ISSN 0004-637X); 410; 2; p. 837-842.
    Format: text
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  • 8
    Publikationsdatum: 2019-07-12
    Beschreibung: The absorption spectrum of Ti I has been analyzed in the region 1900-2315 A. The list contains 219 lines, of which 64 have been identified as transitions between the ground term and terms of the odd configurations (3d + 4s)3 np (n = 4, 5). Sixteen new energy levels have been found, and three odd level values have been revised. Most of the identified Ti I lines are present in the solar spectrum.
    Schlagwort(e): ATOMIC AND MOLECULAR PHYSICS
    Materialart: Physica Scripta (ISSN 0031-8949); 34; 6B D; 759-765
    Format: text
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  • 9
    Publikationsdatum: 2019-07-12
    Beschreibung: Time-resolved observations of the spin-changing, or 'intersystem' emission at 2669.157 A obtained by the ion storage technique are used to measure the transition probability of the 3s2 1S0 - 3s3p 3P1 exp 0 line in Al II. A laser-generated plasma was used as the source of the metastable Al(+) ions. The A-value result obtained for the intersystem transition is 3.33 + or - 0.23 x 10 to the 3rd/sec at the 90-percent confidence level; this value is used to derive two line-intensity ratios which involve the intersystem line as a function of electron density and temperature.
    Schlagwort(e): ATOMIC AND MOLECULAR PHYSICS
    Materialart: Astrophysical Journal, Part 1 (ISSN 0004-637X); 308; 1013-101
    Format: text
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