Publication Date:
1996-08-02
Description:
The molecular mechanisms underlying directed motility of growth cones have not been determined. The role of myosin-V, an unconventional myosin, in growth cone dynamics was examined by chromophore-assisted laser inactivation (CALI). CALI of purified chick brain myosin-V absorbed onto nitrocellulose-coated cover slips inhibited the ability of myosin-V to translocate actin filaments. CALI of myosin-V in growth cones of chick dorsal root ganglion neurons resulted in rapid filopodial retraction. The rate of filopodial extension was significantly decreased, whereas the rate of filopodial retraction was not affected, which suggests a specific role for myosin-V in filopodial extension.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wang, F S -- Wolenski, J S -- Cheney, R E -- Mooseker, M S -- Jay, D G -- NS-29007/NS/NINDS NIH HHS/ -- NS-34699/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1996 Aug 2;273(5275):660-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8662560" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphate/pharmacology
;
Animals
;
Axons/*physiology/ultrastructure
;
Calmodulin-Binding Proteins/antagonists & inhibitors/immunology/*physiology
;
Cells, Cultured
;
Chick Embryo
;
Dendrites/*physiology/ultrastructure
;
Fluorescent Antibody Technique, Indirect
;
Ganglia, Spinal/cytology
;
Lasers
;
Microinjections
;
Myosin Light Chains/antagonists & inhibitors/immunology/*physiology
;
*Myosin Type V
;
Nerve Tissue Proteins/antagonists & inhibitors/immunology/*physiology
;
Pseudopodia/*physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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