Publication Date:
2010-02-27
Description:
CKAMP44, identified here by a proteomic approach, is a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines. CKAMP44 expressed in Xenopus oocytes reduced GluA1- and A2-mediated steady-state currents, but did not affect kainate- or N-methyl-D-aspartate (NMDA) receptor-mediated currents. Mouse hippocampal CA1 pyramidal neurons expressed CKAMP44 at low abundance, and overexpression of CKAMP44 led to stronger and faster AMPA receptor desensitization, slower recovery from desensitization, and a reduction in the paired-pulse ratio of AMPA currents. By contrast, dentate gyrus granule cells exhibited strong CKAMP44 expression, and CKAMP44 knockout increased the paired-pulse ratio of AMPA currents in lateral and medial perforant path-granule cell synapses. CKAMP44 thus modulates short-term plasticity at specific excitatory synapses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉von Engelhardt, Jakob -- Mack, Volker -- Sprengel, Rolf -- Kavenstock, Netta -- Li, Ka Wan -- Stern-Bach, Yael -- Smit, August B -- Seeburg, Peter H -- Monyer, Hannah -- New York, N.Y. -- Science. 2010 Mar 19;327(5972):1518-22. doi: 10.1126/science.1184178. Epub 2010 Feb 25.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Clinical Neurobiology, University of Heidelberg, 6910 Heidelberg, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20185686" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
CA1 Region, Hippocampal/metabolism
;
Calcium Channels/metabolism
;
Dendritic Spines/metabolism
;
Dentate Gyrus/cytology/*metabolism
;
Excitatory Postsynaptic Potentials
;
Glutamic Acid/metabolism
;
Guanylate Kinase
;
Intracellular Signaling Peptides and Proteins/metabolism
;
Membrane Proteins/metabolism
;
Mice
;
Mice, Knockout
;
Miniature Postsynaptic Potentials
;
Molecular Sequence Data
;
Nerve Tissue Proteins/chemistry/genetics/*metabolism
;
Neural Inhibition
;
*Neuronal Plasticity
;
Neurons/*metabolism
;
Oocytes/metabolism
;
Patch-Clamp Techniques
;
Perforant Pathway
;
Protein Interaction Domains and Motifs
;
Protein Isoforms/genetics/metabolism
;
Proteomics
;
Pyramidal Cells/metabolism
;
Receptors, AMPA/chemistry/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
Reverse Transcriptase Polymerase Chain Reaction
;
Synapses/*physiology
;
*Synaptic Transmission
;
Xenopus laevis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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