Publication Date:
1994-12-09
Description:
The stereochemical specificities and reaction courses for both self-splicing steps of a group II intron have been determined by phosphorothioate substitution at the 5' and 3' splice site phosphodiester bonds. Both steps of the splicing reaction proceeded with a phosphorothioate in the Sp configuration but were blocked by the Rp diastereomer. Both steps also proceeded with inversion of stereochemical configuration around phosphorus, consistent with a concerted transesterification reaction. These results are identical to those found for nuclear precursor mRNA (pre-mRNA) splicing and provide support for the hypothesis that group II introns and nuclear pre-mRNA introns share a common evolutionary history.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Padgett, R A -- Podar, M -- Boulanger, S C -- Perlman, P S -- GM31480/GM/NIGMS NIH HHS/ -- GM45371/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1994 Dec 9;266(5191):1685-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas 75235.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7527587" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Exons
;
*Introns
;
Molecular Sequence Data
;
*Nucleic Acid Conformation
;
Oligoribonucleotides/chemistry
;
Oxygen/chemistry
;
Phosphorus/chemistry
;
RNA/*chemistry/genetics
;
*RNA Splicing
;
Sulfur/chemistry
;
Thionucleotides/chemistry
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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