Publication Date:
1998-10-17
Description:
CD8+ cytotoxic T lymphocytes (CTLs) are critical for protection against intracellular pathogens but often have been difficult to induce by subunit vaccines in animals. DNA vaccines elicit protective CD8+ T cell responses. Malaria-naive volunteers who were vaccinated with plasmid DNA encoding a malaria protein developed antigen-specific, genetically restricted, CD8+ T cell-dependent CTLs. Responses were directed against all 10 peptides tested and were restricted by six human lymphocyte antigen (HLA) class I alleles. This first demonstration in healthy naive humans of the induction of CD8+ CTLs by DNA vaccines, including CTLs that were restricted by multiple HLA alleles in the same individual, provides a foundation for further human testing of this potentially revolutionary vaccine technology.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wang, R -- Doolan, D L -- Le, T P -- Hedstrom, R C -- Coonan, K M -- Charoenvit, Y -- Jones, T R -- Hobart, P -- Margalith, M -- Ng, J -- Weiss, W R -- Sedegah, M -- de Taisne, C -- Norman, J A -- Hoffman, S L -- New York, N.Y. -- Science. 1998 Oct 16;282(5388):476-80.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Malaria Program, Naval Medical Research Institute, Bethesda, MD 20889-5607, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9774275" target="_blank"〉PubMed〈/a〉
Keywords:
Adult
;
Animals
;
Antigens, Protozoan/genetics/immunology
;
Female
;
Genes, MHC Class I
;
HLA Antigens/genetics
;
Humans
;
Immunization Schedule
;
Malaria Vaccines/genetics/*immunology
;
Male
;
Plasmodium falciparum/genetics/*immunology
;
Protozoan Proteins/*genetics/*immunology
;
T-Lymphocytes, Cytotoxic/*immunology
;
Vaccination
;
Vaccines, DNA/*immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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