Publikationsdatum:
1990-08-03
Beschreibung:
High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species. Multiple rounds exponentially enrich the population for the highest affinity species that can be clonally isolated and characterized. In particular one eight-base region of an RNA that interacts with the T4 DNA polymerase was chosen and randomized. Two different sequences were selected by this procedure from the calculated pool of 65,536 species. One is the wild-type sequence found in the bacteriophage mRNA; one is varied from wild type at four positions. The binding constants of these two RNA's to T4 DNA polymerase are equivalent. These protocols with minimal modification can yield high-affinity ligands for any protein that binds nucleic acids as part of its function; high-affinity ligands could conceivably be developed for any target molecule.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tuerk, C -- Gold, L -- GM 19963/GM/NIGMS NIH HHS/ -- GM 28685/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1990 Aug 3;249(4968):505-10.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular, Cellular, Developmental Biology, University of Colorado, Boulder 80309.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2200121" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Base Sequence
;
*Biological Evolution
;
DNA-Directed DNA Polymerase/*metabolism
;
Escherichia coli/*enzymology
;
*Genes, Viral
;
Genetic Techniques
;
Ligands
;
*Models, Genetic
;
Molecular Sequence Data
;
Nucleic Acid Conformation
;
Polymerase Chain Reaction
;
RNA, Messenger/genetics/*metabolism
;
RNA, Viral/genetics/*metabolism
;
T-Phages/*enzymology
;
Transcription, Genetic
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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